Dehydrated culture media
Tryptose Broth
For the cultivation of a wide range of fastidious microorganisms, particularly Brucella.
- Cat. No.
- LC-1322
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Tryptose Broth is used for the cultivation of Brucella and other fastidious microorganisms.
Key features
Applications
Enrichment of Brucella.
Preparation
26 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
The media with tryptose, and in this case with thiamine, are recommended for the isolation, cultivation and differentiation of Brucella. Sanders and Huddleson demonstrated that the addition of dextrose and thiamine chlorohydrate to the media stimulates the growth of some species of Brucella.
Tryptose is a source of nitrogen, vitamins and amino acids. Dextrose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance, and thiamine chlorohydrate is a growth factor.
Tryptose Broth with thiamine is also recommended for the cultivation of pathogen microorganisms, for streptococci, pneumococci, meningococci and other fastidious bacteria.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 7.2 ± 0.2 |
| Amount per litre | 26 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 1 |
| Sodium chloride | 5 |
| Thiamine hydrochloride | 0.005 |
| Tryptose | 20 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 26 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
Inoculate and incubate at 35 ± 2 °C under 5-10% of CO₂ for 40-48 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 5-10% CO₂ / 40-48 h.
The microbiological test of the Brucella species should be carried out by the end-user laboratory.
| Microorganism | Specification |
|---|---|
| Streptococcus pyogenesATCC 19615 | Good growth |
| Streptococcus pneumoniaeATCC 6305 | Good growth |
Bibliography
- Jones, L.M., a. Wundt, W.; International Committee on Nomenclature of Bacteria, Subcommittee on the Taxonomy of Brucella. - Int. J. Syst. Bact., 21; 126-128 (1971).
- Picket, M.J., Nelson, E.L., a. Liberman, J.D.; Specification within the Genus Brucella. II. Evaluation of Differential Dye, Biochemical, and Serological Tests. - J. Bact, 66; 210-219 (1953).
- Schindler, R.: Untersuchungen über die Differenzierung von Brucellatypen. - Zbl. Bakt., I. Orig., 164; 93-95 (1955).
- Silverman, S.J., a. Elberg, S.S.; The antigenic relationships of native antigens of species of Brucella. - J. Immunol., 65; 163-174 (1950).
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