Dehydrated culture media
Heart Infusion Broth
General purpose medium used for the growth of fastidious microorganisms.
- Cat. No.
- LC-1323
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Heart Infusion Broth (HIB) is a non-selective, general purpose medium used for the cultivation of many pathogenic and fastidious microorganisms. Highly pathogenic organisms, such as meningococci and pneumococci, can be grown on an infusion medium without enrichments.
Key features
Applications
Enrichment of fastidious microorganisms.
Industry
General cultivation.
Preparation
25 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
The nutritionally rich base of beef heart infusion and tryptose provides nitrogen, vitamins, minerals and amino acids essential for the growth of a variety of microorganisms, and supplies nutritional requirements for the growth of fastidious microorganisms. Sodium chloride supplies essential electrolytes for transport and osmotic balance.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Toasted |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 7.4 ± 0.2 |
| Amount per litre | 25 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Beef heart infusion | 10 |
| Sodium chloride | 5 |
| Tryptose | 10 |
Preparation and use
Preparation
Suspend 25 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Take the inoculum with a sterile loop.
- Submerge the loop into the medium and shake gently.
- Incubate at 35 ± 2 °C for 18-48 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-48 h.
| Microorganism | Specification |
|---|---|
| Streptococcus pyogenesATCC 19615 | Good growth |
| Staphylococcus aureusATCC 25923 | Good growth |
| Streptococcus pneumoniaeATCC 6305 | Good growth |
Bibliography
- Elliott, Kaysner, Jackson and Tamplin. 1995. In FDA bacteriological analytical manual, 8th ed. AOAC International, Gaithersburg, Md.
- Vanderzant and Splittstoesser (ed.). 1992. Compendium of methods for the microbiological examination of foods, 3rd ed. American Public Health Association, Washington, D.C.
- Atlas. 1997. Handbook of microbiological media, 2nd ed. CRC Press, Inc., Boca Raton, Fla.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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Frequently asked questions
Can I ask for formats other than those listed?
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