Ready-to-use media
Mossel EE Broth EP/USP
Liquid culture medium used for the enrichment of enterobacteria according to the ISO standard and the Pharmacopeial Harmonised Method.
- Cat. No.
- LC-4043
- Category
- Ready-to-use media
- Formats
- 2 formats
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 4 months
- Standards
- European PharmacopoeiaUSP
- Also available
- Dehydrated medium, 1 format (LC-1202)
As the name suggests, this medium is for the enrichment of enterobacteria, and is a modification by Mossel (1963) of the classic Brilliant Green Bile 2% Broth. Substitution of lactose by glucose makes it more suitable for enteric bacteria detection (including both gas and non-gas producers) in food and other samples.
Key features
Applications
Selective enrichment of enterobacteria.
Industry
Food, pharmaceutical/veterinary.
Standards
European Pharmacopoeia, USP.
Testing
Growth Promotion Test according to harmonized pharmacopoeial monographs and ISO 11133:2014.
Description and technique
Technique
The most common technique is as follows: the sample to be studied is added to sterile broth in a proportion of 10%. After thorough homogenization, the mixture is incubated for a period of 24-48 hours at 30-35 °C.
After incubation, subcultures are performed on a solid medium appropriate for the selective isolation of enterobacteria (18-24 h).
For this step, Violet Red Bile Glucose Agar is recommended, although MacConkey, VRBLA, deoxycholate or brilliant green based media can also be used.
Presumptive colonies isolated on this medium can be verified following the usual methodology.
Note: temperatures or culture media may vary according to the standards adopted by the laboratory.
Presentation
| Code | Presentation | Filling | Packaging |
|---|---|---|---|
| LC-4043 | 20 tubes, 16 x 113 mm | 9 ± 0.1 mL | 16 x 113 mm glass tubes, ink labelled, metal non-injectable cap. 20 tubes per box. |
| LC-5168 | 10 prepared bottles, 125 mL | 100 ± 3 mL | 1 box with 10 bottles 125 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
Composition
| Ingredient | g/L |
|---|---|
| Gelatin peptone | 10.000 |
| Dextrose | 5.000 |
| Ox bile | 20.000 |
| Disodium phosphate (2H₂O) | 8.000 |
| Monopotassium phosphate | 2.000 |
| Brilliant green | 0.015 |
LC-5168, bottles
| Ingredient | g/L |
|---|---|
| Gelatin peptone | 10.000 |
| Glucose monohydrate | 5.000 |
| Dehydrated ox bile | 20.000 |
| Disodium phosphate (2H₂O) | 8.000 |
| Monopotassium phosphate | 2.000 |
| Brilliant green | 0.015 |
Quality control
Physical/chemical control
| Color | Dark green (LC-5168: green) |
|---|---|
| pH | 7.2 ± 0.2 at 25 °C |
Microbiological control
Growth Promotion Test according to harmonized pharmacopoeial monographs and test methods & ISO 11133:2014.
Aerobiosis. Incubation at 30-35 °C. Reading at 18-24 h.
| Microorganism | Growth |
|---|---|
| Ps. aeruginosaATCC 9027, WDCM 00026 | Good. Recovery in VRBG |
| Salmonella typhimuriumATCC 14028, WDCM 00031 | Good. Recovery in VRBG |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Inhibited |
| Escherichia coliATCC 8739, WDCM 00012 | Good. Recovery in VRBG |
| Enterococcus faecalisATCC 19433, WDCM 00009 | Inhibited |
LC-5168, bottles: microbiological control
Inoculate: 10-100 CFU according to Eur. Pharm. & 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity) according to ISO.
Analytical methodology according to ISO 11133:2014/Amd 1:2018; A2:2020.
Aerobiosis. Incubation: 30-35 °C, reading at 24 h (E.P.) / 37 ± 1 °C, reading at 24 h (ISO).
Note: results ATCC 8739/6538/9027 (30-35 °C) (EP) & ATCC 8739/14028/19433 (37 °C) (ISO).
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 8739, WDCM 00012 | Good. Recovery in VRBG |
| Ps. aeruginosaATCC 9027, WDCM 00026 | Good. Recovery in VRBG |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Inhibited |
| Enterococcus faecalisATCC 19433, WDCM 00009 | Inhibited |
| Mixture E. coliATCC 8739 + E. faecalis ATCC 19433 | > 10 CFU. Characteristic colonies of E. coli |
| Mixture S. typhimuriumATCC 14028 + E. faecalis ATCC 19433 | > 10 CFU. Characteristic colonies of Salmonella |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- EUROPEAN PHARMACOPOEIA 8.0 (2014) 8th ed. § 2.6.13. Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. EDQM. Council of Europe. Strasbourg.
- ISO 21528-1:2004 Standard. Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 1: Detection and enumeration by MPN technique with pre-enrichment.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- MOSSEL, VISSER & CORNELISSEN (1963) The examination of foods for Enterobacteriaceae using a test of the type generally adopted for the detection of salmonellae. J. Appl. Bact. 26:444-452.
- PASCUAL ANDERSON, Mª R. (1992) Microbiología Alimentaria. Díaz de Santos. S.A. Madrid.
- USP 33 - NF 28 (2011) <62> Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. USP Corp. Inc. Rockville. MD. USA.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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