Dehydrated culture media
Motility Test Medium
For the detection of motility of bacteria.
- Cat. No.
- LC-2116
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Motility Test Medium is a semisolid medium used for the detection of bacterial motility. The use of semi-solid media for the determination of bacterial motility eliminates the shortcomings of microscopic methods like the hanging-drop technique.
Key features
Applications
Detection (general use).
Industry
Antimicrobial susceptibility testing, general cultivation.
Preparation
20 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Tryptose is the source of nitrogen, carbon, nutrients and amino acids essential for growth. Sodium chloride maintains the osmotic equilibrium of the medium. A small amount of agar helps to create a semisolid medium.
Inoculate by the stab method with a straight needle. The motility is manifested macroscopically by a diffuse zone of growth spreading from the line of inoculation. Non-motile bacteria generally give growths that are confined to the stab line and have sharply defined margins.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 7.2 ± 0.2 |
| Amount per litre | 20 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 5 |
| Sodium chloride | 5 |
| Tryptose | 10 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 20 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into tubes and sterilize in autoclave at 121 °C for 15 minutes. Cool the tubes in vertical position.
Instructions for use
Agar stab method:
- Pick up the sample with a sterile needle.
- Stab the medium with the sterile needle until it is 1 cm from the bottom of the tube.
- Carefully remove the inoculation needle.
- Incubate at 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification | Characteristic reaction |
|---|---|---|
| Klebsiella aerogenesATCC 13048 | Good growth | Motility (+) |
| Salmonella enteritidisATCC 13076 | Good growth | Motility (+) |
| Klebsiella pneumoniaeATCC 13883 | Good growth | Motility (-) |
| Escherichia coliATCC 25922 | Good growth | Motility (+) |
| Staphylococcus aureusATCC 25923 | Good growth | Motility (-) |
Bibliography
- Tittsler, R.P., and L.A. Sandholzer. 1936. The use of semi-solid agar for the detection of bacterial motility. J. Bacteriol. 31:575-580.
- Green, R.A., E. F. Blum, C. T. DeCoro, R. B. Fairchild, M.T. Kaplan, J. T. Landau and T. S. Sharp. 1951. Rapid methods for the detection of motility. J. Bacteriol. 62:347
- Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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