Dehydrated culture media
Streptococcus Selective Agar Modified (without Neomycin without Colistin)
For isolation of Streptococcus species.
- Cat. No.
- LC-2120
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Minimum order
- 30 packs
Streptococcus Selective Agar Modified (without Neomycin without Colistin) is a medium used for the isolation of Streptococcus species. Streptococci are commonly isolated from the upper respiratory tract. They are also often isolated from burns and other sites where frequently there is an abundance of competing organisms.
Key features
Applications
Selective isolation of Streptococcus.
Industry
Clinical.
Preparation
43.50 g in one liter of distilled water; boil for one minute, avoid overheating, do not autoclave.
Principles and uses
Enzymatic digest of casein and soy peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Organisms positive for esculin hydrolysis hydrolyze the glycoside esculin to esculetin and dextrose. The esculetin reacts with the ferroammonium citrate to form a dark brown or black colony. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.3 ± 0.2 |
| Amount per litre | 43.50 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 17 |
| Casein peptone | 15 |
| Esculin | 1 |
| Ferric ammonium citrate | 0.5 |
| Sodium chloride | 5 |
| Soy peptone | 5 |
| Oxalic acid | 0.005 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 43.50 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. AVOID OVERHEATING. DO NOT AUTOCLAVE.
Instructions for use
- Inoculate the sample as soon as possible after it is received in the laboratory. To culture a specimen from a swab, inoculate the medium by rolling the swab on the agar surface. Inoculate other types of samples by streaking the medium with a sterilized inoculating loop.
- Incubate plates in 5-10% CO₂ at 35 ± 2 °C for 24-48 hours.
- Examine for typical colony morphology and beta hemolysis.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C, 5-10% CO₂ / 24-48 h.
| Microorganism | Specification |
|---|---|
| Streptococcus pyogenesATCC 19615 | Good growth |
| Staphylococcus aureusATCC 25923 | Good growth |
Bibliography
- P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover and R.H. Yolken (eds) Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D.C.
- Petts DN. Colistin-oxolinic acid-blood agar: a new selective medium for streptococci. J Clin Microbiol. 1984;19:4-7.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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Frequently asked questions
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