Dehydrated culture media
Nocive Brewers Bacteria Broth Base Modified
Selective medium for the detection of contaminating and spoilage microorganisms in brewery.
- Cat. No.
- LC-1440
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Nocive Brewers Bacteria Broth Base Modified is a medium used to detect the contaminating and spoilage bacteria in brewery.
Key features
Applications
Detection of microorganisms of the brewing industry.
Industry
Alcoholic beverages.
Preparation
51.3 g in 500 mL of distilled water and 500 mL of beer without gas; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
This medium contains a wide variety of nutrients such as pancreatic digest of casein, yeast extract, meat extract, dextrose and maltose. These nutrients enhance the growth of this type of microorganisms.
Potassium acetate (instead of sodium acetate) makes the medium less inhibitory to the growth of bacteria that deteriorate beer and other beverages. Polysorbate 80 is incorporated to neutralize phenols, hexachlorophene and formalin. L-Cysteine is the reducing agent. Disodium phosphate acts as a buffer system.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Pink-red |
| Final pH (25 °C) | 5.8 ± 0.2 |
| Amount | 51.3 g in 500 mL of distilled water and 500 mL of beer without gas |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 15 |
| Beef extract | 2 |
| Disodium phosphate | 2 |
| Maltose | 15 |
| Pancreatic digest of casein | 5 |
| Polysorbate 80 | 0.5 |
| Yeast extract | 5 |
| L-Malic acid | 0.5 |
| L-Cysteine HCl | 0.2 |
| Chlorophenol red | 0.07 |
| Potassium acetate | 6 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 51.3 grams of the medium in 500 mL of distilled water and 500 mL of beer without gas. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C, mix well and dispense into tubes.
Instructions for use
Incubate at 30-35 °C and observe after 4 days.
After incubation, the isolated acid-producing colonies must be tested by Gram stain and catalase tests. Gram-positive and catalase-negative cocci or rods may tentatively be considered lactic acid bacteria.
Quality control
Microbiological test
Incubation conditions: 30-35 °C / 4 days.
| Microorganism | Specification | Characteristic reaction |
|---|---|---|
| Pediococcus damnosusATCC 29358 | Good growth | Acid production (yellow) |
| Pediococcus acidilacticiATCC 8042 | Good growth | Acid production (yellow) |
| Lactobacillus brevisATCC 8287 | Good growth | Acid production (yellow) |
Additional information
Due to the composition of the medium and its hygroscopic properties, the powder could appear lumpy and moist. This phenomenon does not affect the microbiological performance of the medium.
Bibliography
- Back, 1980 Brauwelt 1562
- Dacha, 1981, Brauwelt 1778
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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