Dehydrated culture media
Wort Broth
For the cultivation and enumeration of yeasts.
- Cat. No.
- LC-1444
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Wort Broth is commonly used for the detection and enumeration of yeasts and fungi, particularly yeasts in butter, syrups and other materials, especially in the soft drinks industry.
Key features
Applications
Detection of yeasts and molds and selective enumeration of yeasts.
Industry
Food.
Preparation
33 g in one liter of distilled water, with 2-3 mL of glycerol; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Yeasts grow well on media that contain dextrose or maltose, even more so if the reaction is acidic. This medium has been enriched with the addition of salts and other nutrients. The low pH inhibits the growth of most bacteria.
Malt extract and casein peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Dextrin is a carbon source. Dipotassium phosphate is the buffer. Glycerol limits bacterial growth by reducing the water activity from 0.999 to 0.95.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 4.8 ± 0.2 |
| Amount per litre | 33 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Ammonium chloride | 1 |
| Casein peptone | 1 |
| Dipotassium phosphate | 1 |
| Malt extract | 15 |
| Dextrin | 2.5 |
| D-Maltose | 12.5 |
Preparation and use
Preparation
Suspend 33 grams of the medium in one liter of distilled water. Add 2-3 mL of glycerol. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
Incubate at 30 °C and observe after 40-48 hours.
Quality control
Microbiological test
Incubation conditions: 30 °C / 40-48 h.
| Microorganism | Specification |
|---|---|
| Aspergillus brasiliensisATCC 16404 | Good growth |
| Saccharomyces cerevisiaeATCC 9080 | Good growth |
| Saccharomyces cerevisiaeATCC 9763 | Good growth |
Bibliography
- J. Dairy Science, 16: 141.
- RAPP Milchwiss, 29, 341-344 (1974).
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
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