Ready-to-use media
Potato Dextrose Agar with Chloramphenicol ISO
Culture medium used for the detection and enumeration of fungi in food.
- Cat. No.
- LC-947
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- ISO 17025 quality control
- Yes
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Minimum order
- 15 packs
- Standards
- ISO 16212
Potato Dextrose Agar is a weakly selective medium for fungi due to its high sugar content, acidic pH, and the antibiotic (chloramphenicol) presence.
Key features
Applications
Selective enumeration of yeasts and molds.
Industry
Cosmetics, food, pharmaceutical/veterinary.
Standards
ISO 16212.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Description and technique
Description
Pigment production and aerial mycelium development are enhanced by the potato peptone, especially in Fusarium, Aspergillus and Penicillium species.
Technique
The plates are inoculated by standard methods, according to the protocols established for each type of sample in each control laboratory. They are incubated for 3-5 days at 30 ± 1 °C to permit the complete development of the fungal (molds and yeasts) colonies.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Potato peptone (1) | 4.00 |
| Glucose | 20.000 |
| Chloramphenicol | 0.050 |
| Agar | 15.000 |
(1) Equivalent to 200 g infusion from potatoes.
Quality control
Physical/chemical control
| Color | Yellowish |
|---|---|
| pH | 5.6 ± 0.2 at 25 °C |
Microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ (selectivity).
Aerobiosis. Incubation at 30 ± 1 °C. Reading at 48 h - 5 days.
| Microorganism | Growth |
|---|---|
| Aspergillus brasiliensisATCC 16404, WDCM 00053 | Good |
| Candida albicansATCC 10231, WDCM 00054 | Good |
| Escherichia coliATCC 8739, WDCM 00012 | Inhibited |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Inhibited |
Sterility control
Incubation 48 h at 30-35 °C and 48 h at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS R.M. (1995) Handbook of Microbiological Media for the Examination of Food. CRC Press. Boca Raton. Florida. USA.
- EUROPEAN PHARMACOPOEIA 8.0 (2014) 8th ed. § 2.6.13. Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. EDQM. Council of Europe. Strasbourg.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 16212 Standard (2017) Cosmetics - Microbiology - Enumeration of yeast and mould.
- RICHARDSON, G.H. (1985) Standard Methods for the examination of dairy products 15th ed. APHA. Washington.
- USP 33 - NF 28 (2011) <62> Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. USP Corp. Inc. Rockville. MD. USA.
- VANDERZANT, C. & D.F. SPLITTSTOESSER (1992) Compendium of methods for the microbiological examination of foods. 3rd ed. APHA. Washington.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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