Ready-to-use media
Eugon LT Modified Agar ISO
Solid medium used for the detection and enumeration of aerobic mesophilic bacteria, in cosmetic products with and without preservatives.
- Cat. No.
- LC-953
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- ISO 17025 quality control
- Yes
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Minimum order
- 15 packs
Modification of the Eugon LT 100 medium, in which Triton X-100 (Octoxynol 9) has been replaced by sodium lauryl sulfate and the concentration of polysorbate 80 has been increased to maintain dispersing capacity.
Key features
Applications
Detection of mesophilic aerobes and enumeration with neutralizers.
Industry
Cosmetics.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Packaging
1 box with 2 packs of 10 plates/pack. Single cellophane.
Description and technique
Technique
A 1:10 dilution of the sample is prepared using Eugon broth if the sample is soluble in water. If the sample is non-water soluble, it must be emulsified with a suitable agent (e.g. polysorbate 80). Once emulsified, the sample is added to a suitable volume of Eugon broth (e.g. 1:10). The pour plate or spread plate technique can be adopted with unfilterable samples.
If the sample is filterable, it is recommended to filter it through a membrane filter with a nominal pore size no greater than 0.45 µm and to wash it with defined volumes of water or diluent (Universal Diluent). Immediately transfer the membrane to a plate of Eugon Agar.
The inoculated plates are incubated at 32.5 ± 2.5 °C for 48 to 72 hours and the colonies are then counted.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Tryptone | 15.00 |
| Soy peptone | 5.00 |
| Polysorbate 80 | 15.00 |
| Dextrose | 5.50 |
| Sodium chloride | 4.00 |
| Sodium lauryl sulfate | 1.56 |
| Lecithin | 1.00 |
| L-Cystine | 0.70 |
| Sodium sulfite | 0.20 |
| Agar | 15.00 |
Quality control
Physical/chemical control
| Color | Straw-coloured yellow |
|---|---|
| pH | 7.0 ± 0.2 at 25 °C |
Microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity).
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Analytical methodology according to ISO 11133:2014/Amd 1:2018; A2:2020.
Aerobiosis. Incubation at 32.5 ± 2.5 °C, reading after 24-48 h.
| Microorganism | Growth |
|---|---|
| Stph. epidermidisATCC 12228, WDCM 00036 | Good (≥ 70%) |
| Salmonella typhimuriumATCC 14028, WDCM 00031 | Good (≥ 70%) |
| Bacillus subtilisATCC 6633, WDCM 00003 | Good (≥ 70%) |
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 70%) |
| Aspergillus brasiliensisATCC 16404 | Good (≥ 70%) |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Good (≥ 70%) |
| Escherichia coliATCC 8739, WDCM 00012 | Good (≥ 70%) |
| Ps. aeruginosaATCC 9027, WDCM 00026 | Good (≥ 70%) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- GUISNO, R., I.W. GIBBY & M.J. FOTER (1946) A neutralizing medium for evaluation of the germicidal potency of the quaternary ammonium salts. Amer. J. Pharm. 118:320-323.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 21149 Standard (2017) Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria.
- ISO 21149 (2017)/Amd 1:2021 Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria.
- ISO 21150 Standard (2015) Cosmetics - Microbiology - Detection of Escherichia coli.
- ISO 22717 Standard (2015) Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa.
- ISO 22718 Standard (2015). Cosmetics - Microbiology - Detection of Staphylococcus aureus.
- WILLIAMSON, P. & A.M. KLIGMAN (1965) A new method for the quantitative investigation of cutaneous bacteria. J. Inv. Dermatol. 45:498-503.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.