Ready-to-use media
TSC Agar with Egg Yolk
Medium recommended for isolation, cultivation and enumeration of Clostridium perfringens according to ISO standards.
- Cat. No.
- LC-964
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Standards
- ISO 14189ISO 7937
The medium is a modification of the classical TSN Agar in which the traditional antibiotics, polymyxin and neomycin, have been replaced by cycloserine. Cycloserine has been found more selective for Clostridium perfringens, and reduces the production of diffuse blackening. Clostridium perfringens is more resistant to cycloserine than to sulfadiazine, polymyxin and neomycin, hence reducing the dosage. The presence of sodium meta-bisulfite and ferric ammonium citrate allows three differential characteristics of this anaerobic species to be verified with just one assay. These characteristics are sulfite reduction, growth at 44 ± 1 °C and cycloserine resistance.
Key features
Applications
Detection and selective enumeration of Clostridium perfringens.
Industry
Food, water.
Standards
ISO 14189, ISO 7937.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Description and technique
Technique
Collect, dilute and prepare samples and volumes to be filtered as required according to specifications, directives, official standard regulations and/or expected results.
Inoculate the sample and cover it with a second layer of room temperature melted agar.
Incubate the plates anaerobically at 44 ± 1 °C for 21 ± 3 h.
(Incubation times greater than those mentioned above or different incubation temperatures may be required depending on the sample, on the specifications, etc.)
After incubation, enumerate the colonies with a black iron sulfide precipitate (H₂S+).
Confirmation of characteristic colonies as C. perfringens is required, through further microbiological or biochemical tests.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Tryptone | 15.0 |
| Soy peptone | 5.0 |
| Yeast extract | 5.0 |
| Sodium meta-bisulfite | 1.0 |
| Ferric ammonium citrate | 1.0 |
| Cycloserine | 0.4 |
| Egg yolk emulsion | 50.0 mL |
| Agar | 18.0 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|---|
| pH | 7.6 ± 0.2 at 25 °C |
Microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ (selectivity).
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Anaerobiosis. Incubation at 44 ± 1 °C for 21 ± 3 h.
| Microorganism | Growth |
|---|---|
| Clostridium perfringensATCC 13124, WDCM 00007, NCTC 8237 | Good (≥ 50%) |
| Bacillus subtilisATCC 6633, WDCM 00003 | Inhibited |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M., L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- DIN Standard 10165. Referenz Verfahren fur Bestimmung von Clostridium perfringens. Fleisch und Fleischerzeugnissen.
- DOWNES, F.P. & K. ITO (2001) Compendium of Methods for the Microbiological Examination of Foods. 4th ed. American Public Health Association. Washington.
- DIRECTIVA 2015/1787/UE de la Comisión por la que se modifica la Directiva 98/83/CE relativa a la calidad de las aguas destinadas al consumo humano (DO L260 de 7.10.2015 pg 6 y ss).
- FDA (Food and Drug Administration) (1998) Bacteriological Analytical Manual. 8th ed. Rev. A. AOAC International Inc. Gaithersburg. MD.
- ISO 7937 (2004) Microbiology of Food and Animal Feeding Stuffs. Horizontal Method for Enumeration of C. perfringens. Colony-count technique.
- ISO 6461-2 (1986) Water Quality - Detection and enumeration of the spores of sulfite-reducing anaerobes (Clostridia) - Part 2: Method by Membrane Filtration.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 14189 (2013) Water quality. Enumeration of Clostridium perfringens - Method using membrane filtration.
- SMITH, L.D. (1981) Clostridial Anaerobic Infections, in Diagnostic Procedures for Bacterial Mycotic and Parasitic Infections. 6th ed. APHA. Washington.
- UNE-EN ISO 11133 (2014). Microbiología de los alimentos para consumo humano, alimentación animal y agua - Preparación, producción, conservación y ensayos de rendimiento de los medios de cultivo.
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The product
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