Ready-to-use media
Sabouraud Dextrose Agar with Chloramphenicol Slant
Medium for the enumeration and cultivation of fungi (yeast, mold and dermatophytes).
- Cat. No.
- LC-4063
- Category
- Ready-to-use media
- Format
- 20 tubes
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 4 months
- Standards
- European PharmacopoeiaUSP
This culture medium differs from the classical Sabouraud Agar only by the addition of chloramphenicol. This thermostable antibiotic has a broad antibacterial spectrum which ensures the selective isolation of fungi from highly contaminated clinical and non-clinical samples.
Key features
Applications
Selective enumeration of yeasts and molds.
Industry
Food, general cultivation, pharmaceutical/veterinary.
Standards
European Pharmacopoeia, USP.
Presentation
20 slant tubes, 16 x 113 mm, with 6.2 ± 0.3 mL.
Description and technique
Technique
To inoculate tubes, follow the standard laboratory methods or the applicable norms: stab inoculation, loop inoculation, etc.
Incubate the tubes right side up aerobically at 20-25 °C for 48-72 h.
(Incubation times greater than those mentioned above or different incubation temperatures may be required depending on the sample, on the specifications. This medium can be inoculated directly or after treatment of the sample.)
Each laboratory must evaluate the results according to their specifications.
The colonies that appeared on the surface must be confirmed by further microbiological and biochemical tests: dye hyphae and/or sporangium; re-inoculating in a more selective and differential medium, DTM Agar.
Precautions for use
For professional use only.
Do not use if the product is contaminated, broken, or spoiled.
Presentation
| Presentation | 20 slant tubes, 16 x 113 mm |
|---|---|
| Filling | 6.2 ± 0.3 mL |
| Packaging | 1 box with 20 tubes, 16 x 113 mm glass tubes, ink labelled and metal cap. |
Composition
| Ingredient | g/L |
|---|---|
| D(+)-Glucose | 40.00 |
| Peptone from casein | 5.00 |
| Meat peptone | 5.00 |
| Chloramphenicol | 0.50 |
| Agar | 15.00 |
Quality control
Physical/chemical control
| Color | Straw-coloured yellow |
|---|---|
| pH | 5.6 ± 0.2 at 25 °C |
Microbiological control
Loop spreading.
Aerobic. Incubation at 20-25 °C for 24-72 h (bacteria) and up to 3-5 days (moulds and yeast).
| Microorganism | Growth |
|---|---|
| Aspergillus brasiliensisATCC 16404, WDCM 00053 | Good |
| Candida albicansATCC 10231, WDCM 00054 | Good |
| Bacillus subtilisATCC 6633, WDCM 00003 | Inhibited |
| Escherichia coliATCC 8739, WDCM 00012 | Inhibited |
| S. cerevisiaeATCC 9763, WDCM 00058 | Good |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- AJELLO, L. (1957) Cultural Methods for Human Pathogenic Fungi. J. Chron. Dis. 5:545-551.
- COLIPA (1997) Guidelines on Microbial Quality Management (MQM). Brussels.
- EUROPEAN PHARMACOPOEIA 8.0 (2014) 8th ed. § 2.6.13. Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. EDQM. Council of Europe. Strasbourg.
- GEORGE, L.K., AJELLO, L. & PAPAGEORGE, C. (1954) Use of Cycloheximide in the Selective Isolation of Fungi Pathogenic to Man. J. Lab. Clin. Med, 44 (422-428).
- HANTSCHKE, D. (1968) Mykosen, 11, (769-778).
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- PAGANO, J. LEVIN, J.D. and TREJO, W. (1957-58) Diagnostic Medium for Differentiation of Species of Candida. Antibiotics Annual, 137-143.
- SABOURAUD, R. (1910) Les Teignes. Masson, Paris.
- USP 33 - NF 28 (2011) <62> Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. USP Corp. Inc. Rockville. MD. USA.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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