Ready-to-use media
Saline Peptone Water ISO
Isotonic diluent for the maximal recovery of stressed microorganisms according to ISO standards.
- Cat. No.
- LC-4044
- Category
- Ready-to-use media
- Formats
- 2 formats
- Storage
- 8 to 25 °C
- Standards
- ISO 22718ISO 6887ISO 8199
Diluent and non-selective pre-enrichment medium that has the property of revitalization of the peptone.
Key features
Applications
Diluent (general use).
Industry
General cultivation.
Standards
ISO 22718, ISO 6887, ISO 8199.
Testing
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Description and technique
Peptone is the nutrient source of nitrogen, vitamins, amino acids and minerals; sodium chloride maintains the osmotic balance.
Inoculate according to final purpose, samples and validated methods.
Presentation
| Code | Presentation | Filling | Packaging |
|---|---|---|---|
| LC-4044 | 20 tubes, 16 x 113 mm | 9 ± 0.1 mL | 1 box with 20 tubes, 16 x 113 mm glass tubes, ink labelled and metal non-injectable cap. |
| LC-5182 | 10 prepared bottles, 125 mL | 90 ± 3 mL | 1 box with 10 bottles 125 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
Composition
| Ingredient | g/L |
|---|---|
| Sodium chloride | 8.5 |
| Pancreatic digest of casein | 1.0 |
Quality control
Physical/chemical control
| Color | Colourless |
|---|---|
| pH | 7.0 ± 0.2 at 25 °C |
Microbiological control
Prepare tubes / inoculate ≤ 10³ CFU/tube (productivity) / subculture after holding at 20-25 °C for 45 min to 1 h.
Aerobiosis. Incubation at 30-35 °C. Reading at 24-48 h.
Microbiological control according to ISO 11133:2014/Amd 1:2018.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 8739, WDCM 00012 | Good. Recovery ± 30% T0 (original enumeration) |
| Candida albicansATCC 10231, WDCM 00054 | Good. Recovery ± 30% T0 (original enumeration) |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Good. Recovery ± 30% T0 (original enumeration) |
| Ps. aeruginosaATCC 9027, WDCM 00026 | Good. Recovery ± 30% T0 (original enumeration) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ISO 6887-1:1999 Microbiology of food and animal feeding stuffs. Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions - Part 2 (2003): Specific rules for the preparation of meat and meat products.
- ISO 8261:2001 Standard. Milk and milk products - General guidance for the preparation of test samples, initial suspension and decimal dilution for microbiological examination.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 16212 Standard (2017) Cosmetics - Microbiology - Enumeration of yeast and mould.
- ISO 21149 Standard (2017) Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria.
- ISO 21150 Standard (2015) Cosmetics - Microbiology - Detection of Escherichia coli.
- ISO 22717 Standard (2015) Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa.
- ISO 22718 Standard (2015). Cosmetics - Microbiology - Detection of Staphylococcus aureus.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.