Ready-to-use media
Xilose Broth ISO
Liquid medium to verify the utilization of xylose in fastidious microorganisms, such as Listeria spp., according to ISO 11290.
- Cat. No.
- LC-4068
- Category
- Ready-to-use media
- Format
- 20 tubes
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 4 months
- Standards
- ISO 11290
Highly nutritious liquid medium for utilization of sugars, described in ISO 11290-1, for fastidious microorganisms such as Listeria spp. D-xylose is included as carbohydrate.
Key features
Applications
Differentiation of Listeria.
Industry
Food.
Standards
ISO 11290.
Presentation
20 tubes, 16 x 113 mm, with 2 ± 0.1 mL.
Description and technique
Inoculate with colonies from pure cultures grown on a confirmative medium. Incubate at 37 ± 1 °C for 24-48 h. Positive reactions (formation of acid from xylose) are manifested by turning purple to yellow, and this occurs mostly at 24 hours of incubation. The intensity of the yellow color is proportional to the amount of inoculum and acid produced.
Note: Positive reactions may occur at 5 days in macrovolume tubes.
Each laboratory must evaluate the results according to current normatives.
Presentation
| Presentation | 20 tubes, 16 x 113 mm |
|---|---|
| Filling | 2 ± 0.1 mL |
| Packaging | 16 x 113 mm glass tubes, ink labelled, metal non-injectable cap. 20 tubes per box. |
Composition
| Ingredient | g/L |
|---|---|
| Enzymatic digest of animal tissues | 10.00 |
| Meat extract | 1.00 |
| Sodium chloride | 5.00 |
| D-Xylose | 5.00 |
| Bromocresol purple | 0.02 |
Quality control
Physical/chemical control
| Color | Purple |
|---|---|
| pH | 6.8 ± 0.2 at 25 °C |
Microbiological control
Inoculate a pure culture.
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobiosis. Incubation at 37 ± 1 °C, reading after 24/44 h to 5 days.
| Microorganism | Growth |
|---|---|
| L. monocytogenesATCC 13932, WDCM 00021 | D-Xylose (-). Medium does not change color. |
| Listeria ivanoviiATCC 19119 | D-Xylose (+). Medium yellow. |
| Listeria monocytogenesATCC 35152 | D-Xylose (-). Medium does not change color. |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M. and L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press Inc. Boca Raton Fla. USA.
- F.D.A. (1988) Bacteriological Analytical Manual. 8th Edition. Rev. A. AOAC International. Gaithersburg. MD. USA.
- HITCHINS, A.D. and K. JINNERMAN (2013) Detection and enumeration of Listeria monocytogenes in foods. In FDA BAM, Chapter 10.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 11290-1 Standard (2017) Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 1: Detection method.
- ISO 11290-2 Standard (2017) Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 2: Enumeration method.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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