PCR and qPCR mixes
SNP Pol DNA Polymerase 2X PCR Master Mix
Hot-start 2X master mix with high allele discrimination for SNP genotyping
- Cat. No.
- M3061
- Category
- PCR and qPCR mixes
- Formats
- 2 formats
- Manufacturer
A ready-to-use 2X master mix containing the engineered high-discrimination polymerase, an optimised reaction buffer and ultrapure dNTPs with a balanced dUTP/dTTP mix. The polymerase does not extend primers with a 3' mismatch, so only the perfectly matched allele is amplified, and an aptamer keeps it inactive below 55 °C. Just add primers and template, even whole cells, to run allele-specific PCR or real-time PCR with intercalating dyes.
Key features
Up to 100% discrimination
Detects fewer than 10 mutant copies among more than 10,000 wild-type copies in allele-specific PCR.
All in one tube
Premixed polymerase, buffer and dNTPs cut set-up time and pipetting errors.
UDG compatible
The dUTP/dTTP mix supports carry-over contamination control with uracil-DNA glycosylase.
Direct PCR from cells
50-500 animal or E. coli cells are used directly, with no separate lysis or proteinase K.
Applications
- Allele-specific PCR (ASA) and SNP genotyping
- Methylation-specific PCR (MSP)
- HLA typing
- Multiplex PCR
- Verification of CRISPR/Cas9 editing products
- Confirmation of point mutations found by sequencing
- Real-time PCR with intercalating dyes
Technical details
| Concentration | 2X (1X in the final reaction) |
|---|---|
| Composition | High-discrimination polymerase with aptamer hot start, optimised buffer, dNTPs with a balanced dUTP/dTTP mix |
| Hot start | Reversible inhibition below 55 °C; annealing temperature above 57 °C recommended |
| Amplicon length | Optimised for 60-200 bp; above 500 bp extra MgCl2 (0.5-2.0 mM) may be needed |
| Extension rate | 0.5-1 kb/min at 72 °C |
| Activity | No 5'-3' or 3'-5' exonuclease activity |
| Compatibility | Intercalating dyes in real-time PCR; not suitable for hydrolysis probes |
| Storage | -20 °C; up to 3 months at +2/+8 °C |
| Shipping | On wet ice |
| Quality control | ASA test on genomic SNP rs72921001 in HeLa genomic DNA; activity adjusted on an artificial template |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.