DNA polymerases
SNP Pol DNA Polymerase
High-discrimination hot-start DNA polymerase for allele-specific PCR and SNP analysis
- Cat. No.
- M3009
- Category
- DNA polymerases
- Formats
- 2 formats
- Manufacturer
An engineered recombinant DNA polymerase variant that does not extend primers carrying a mismatch at the 3' end, so amplification only occurs on the perfectly matched allele. A standard primer whose 3' nucleotide sits on the point mutation is enough to tell wild type from mutant without sequencing. An aptamer reversibly inhibits the enzyme below 55 °C and prevents non-specific amplification during set-up.
Key features
Allele discrimination
Detects fewer than 10 mutant copies among more than 10,000 wild-type copies, with up to 100% discrimination in allele-specific PCR.
Aptamer hot start
Reversible inhibition below 55 °C; primers with an annealing temperature above 57 °C are recommended.
Direct PCR from cells
Animal cells and E. coli (50-500 cells) go straight into the reaction, with no separate lysis or proteinase K digestion.
Taq-like fidelity
The error rate is comparable to wild-type Taq.
Applications
- Allele-specific PCR (ASA/AS-PCR) and SNP genotyping
- Allele-specific primer extension (AS-PEX)
- Methylation-specific PCR (MSP) after bisulfite treatment, down to single CpG sites
- HLA typing
- Verification of CRISPR/Cas9 editing products
- Confirmation and quantification of mutations found by sequencing or NGS
- Real-time PCR with intercalating dyes
- Minisequencing
Technical details
| Concentration | 5 U/µL |
|---|---|
| Supplied with | Optimised 10x reaction buffer |
| Activity | 5'-3' polymerase; no 5'-3' or 3'-5' exonuclease activity |
| Hot start | Aptamer inhibition, reversed above 55 °C |
| Extension rate | 0.5-1 kb/min at 72 °C |
| Amplicon length | 60-200 bp recommended; above 500 bp extra Mg2+ (0.5-1.5 mM) may be needed |
| Compatibility | Non-specific intercalating dyes in real-time PCR; not suitable for hydrolysis probes |
| Unit definition | 1 U incorporates 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72 °C |
| Storage | -20 °C; up to 9 months at +2/+8 °C |
| Shipping | On wet ice; full activity for at least 2 weeks at +15/+25 °C |
| Quality control | ASA test on genomic SNP rs72921001 in HeLa genomic DNA; activity adjusted on an artificial template; concentration by protein staining; no contamination detected |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.