Dehydrated culture media
TGEA Medium (Tryptone Glucose Yeast Extract Agar)
For total count of aerobic organisms in water, dairy products, etc.
- Cat. No.
- LC-1190
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
TGEA Medium (Tryptone Glucose Yeast Extract Agar) is a medium used for the total count of aerobic mesophilic bacteria in water and dairy products.
Key features
Applications
Selective enumeration of mesophilic aerobes.
Industry
Dairy products, water.
Preparation
28 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Casein peptone and beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly the B-group. Dextrose is the fermentable carbohydrate providing carbon and energy. Bacteriological agar is the solidifying agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Clear amber, slightly opalescent |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 28 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Glucose | 1 |
| Bacteriological agar | 18 |
| Beef extract | 3 |
| Casein peptone | 5 |
| Yeast extract | 1 |
Preparation and use
Preparation
Suspend 28 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
Pour plate technique:
- Inoculate 1 mL of the sample (if necessary 2 continuous decimal dilutions to be able to count between 15-300 colonies per plate).
- Put 12-15 mL per plate of agar cooled to 44-47 °C in each Petri dish. The time of preparation should not exceed 45 minutes.
- Invert the plates and incubate at 35 ± 2 °C for 24 hours.
- Post incubation, count the colonies.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 24 h.
| Microorganism | Specification |
|---|---|
| Bacillus cereusATCC 11778 | Good growth |
| Streptococcus agalactiaeATCC 13813 | Good growth |
| Salmonella typhimuriumATCC 14028 | Good growth |
| Escherichia coliATCC 25922 | Good growth |
| Staphylococcus aureusATCC 25923 | Good growth |
| Pseudomonas aeruginosaATCC 27853 | Good growth |
Bibliography
- Wehr and Frank (ed.). 2004. Standard methods for the examination of dairy products, 17th ed. American Public Health Association, Washington, D.C.
- Bowers and Hucker. 1935. Tech. Bull. 228. NY State Agr. Exp. Sta.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.