Ready-to-use media
TSC with MUP Agar
Solid medium used for the detection and presumptive identification of Clostridium perfringens in various samples.
- Cat. No.
- LC-4744
- Category
- Ready-to-use media
- Format
- 30 water plates, 55 mm
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
TSC-MUP Agar is a primary isolation and indirect confirmation medium for Clostridium perfringens, allowing a presumptive identification in 4-5 hours, compared to the two to three days required by the established traditional methods (MNLG, motility-nitrate-lactose-gelatin).
Key features
Applications
Detection and selective enumeration of Clostridium perfringens.
Industry
Food, water.
Presentation
30 membrane filtration plates, 55 mm, with 9 ± 1 mL.
Packaging
1 box containing 6 plastic bags with 5 plates of 55 mm/bag.
Description and technique
Technique
The sample is collected and processed according to the protocol established by each laboratory for each product, following the institutional, national or international rules and laws.
The membrane through which the processed sample was filtered is carefully deposited on the surface of the TSC-MUP agar, taking care not to trap air bubbles.
Alternatively, a thin layer of molten TSC agar or agar can be used as an overlay on the membrane.
The plates are incubated in anaerobiosis at 44 ± 1.0 °C for 21 ± 3 h; under UV light (365 nm), the fluorescence of presumptive colonies of Cl. perfringens is observed.
Presentation
| Presentation | 30 membrane filtration plates, 55 mm |
|---|---|
| Filling | 9 ± 1 mL |
| Packaging | 1 box containing 6 plastic bags with 5 plates of 55 mm/bag. |
Composition
| Ingredient | g/L |
|---|---|
| Enzymatic digest of casein | 15.00 |
| Soy peptone | 5.00 |
| Yeast extract | 5.00 |
| Sodium meta-bisulfite | 1.00 |
| Ferric ammonium citrate | 1.00 |
| Cycloserine | 0.40 |
| M.U.P. | 0.10 |
| Agar | 18.00 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|---|
| pH | 7.6 ± 0.2 at 25 °C |
Microbiological control
Membrane filtration / practical range 100 ± 20 CFU, min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity) / ≥ 10³ CFU (specificity).
Analytical methodology according to ISO 11133:2014/Amd 1:2018; A2:2020.
Anaerobiosis. Incubation at 44 ± 1 °C for 21 ± 3 h.
Microbiological control according to ISO 11133:2014/Amd 1:2018.
| Microorganism | Growth |
|---|---|
| Clostridium perfringensATCC 13124, WDCM 00007, NCTC 8237 | Good ≥ 50% - black colonies - fluorescent |
| Clostridium perfringensATCC 10543, WDCM 00174 | Good ≥ 50% - black colonies - fluorescent |
| Bacillus subtilisATCC 6633, WDCM 00003 | Inhibited |
| Clostridium sporogenesATCC 11437 | Good - NO fluorescence |
A double layer with TSC agar favors the observation of the blackening of the H₂S (+) strains.
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ADCOCK, P.W. & C.P. SAINT (2001) Rapid Confirmation of Clostridium perfringens by Using Chromogenic and Fluorogenic Substrates. App. Environm. Microbiol. 67(9):4382-4384.
- ARAUJO, M., R.A. SUEIRO, M.J. GÓMEZ & M.J. GARRIDO (2001) Evaluation of fluorogenic TSC agar for recovering Clostridium perfringens in groundwater samples. Water Sci. Technol. 43:201-204.
- ARAUJO, M., R.A. SUEIRO, M.J. GÓMEZ & M.J. GARRIDO (2004) Enumeration of Clostridium perfringens spores in groundwater samples: comparison of six culture media. J. Microbiol. Methods 57:175-180.
- SARTORY, D.P., R. WALDOCK, C.E. DAVIES & A.M. FIELD (2006) Evaluation of acid phosphatase as confirmation test for Clostridium perfringens isolated from water. Letter App. Microbiol. 42:418-424.
- ISO 7937:2004. Microbiology of Food and Animal Feeding Stuffs. Horizontal Method for Enumeration of C. perfringens. Colony-count technique.
- ISO 6461-2:1986 Water Quality - Detection and enumeration of the spores of sulfite-reducing anaerobes (Clostridia) - Part 2: Method by Membrane Filtration.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 14189:2013. Water quality. Enumeration of Clostridium perfringens - Method using membrane filtration.
- ISO 15213:2003. Standard. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of sulfite-reducing bacteria growing under anaerobic conditions.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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