Ready-to-use media
Eugon LT Modified Broth
Liquid medium used for the enrichment of aerobic bacteria including E. coli, in cosmetic products with and without preservatives.
- Cat. No.
- LC-5066
- Category
- Ready-to-use media
- Formats
- 3 formats
- ISO 17025 quality control
- Yes
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 4 months
Modification of the Eugon LT 100 medium, in which Triton X-100 (Octoxynol 9) has been replaced by sodium lauryl sulfate and the concentration of polysorbate 80 has been increased to maintain dispersing capacity.
Key features
Applications
Enrichment of mesophilic aerobes and selective enumeration of Pseudomonas.
Industry
Cosmetics.
Testing
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Description and technique
Technique
Prepare a 1:10 dilution of the sample using Eugon broth directly. If the sample is not water-miscible, a suitable suspension should be prepared with a suitable dispersing agent and then diluted in a suitable amount of Eugon broth (e.g. 1:10 or 1:50).
If the sample is filterable, it is recommended to filter it through a membrane with a nominal pore not greater than 0.45 µm and wash it with defined volumes of water or diluents (Maximum Recovery Diluent). The membrane is immediately transferred to a container containing a suitable volume of the Eugon broth. The broth inoculated either with the sample, its dispersion or with the membrane is incubated at 32.5 ± 2.5 °C for at least 20 hours and at most 72 hours.
If what is desired is to have an estimate of the population by the MPN method, proceed as follows:
Prepare a decimal bank of dilutions from the product under examination. Inoculate the tubes or containers of each series with the established volume and incubate them according to the temperatures and times standardized in the analytical protocol applied. The enumeration will be done according to the tables of the Most Probable Number that are applicable in each case.
Presentation
| Code | Presentation | Filling | Packaging |
|---|---|---|---|
| LC-5066 | 10 prepared bottles, 125 mL | 100 ± 3 mL | 1 box with 10 bottles 125 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
| LC-5067 | 10 prepared bottles, 250 mL | 200 ± 3 mL | 1 box with 10 bottles 250 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
| LC-5068 | 10 prepared bottles, 125 mL | 90 ± 3 mL | 1 box with 10 bottles 125 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
Composition
| Ingredient | g/L |
|---|---|
| Tryptone | 15.00 |
| Soy peptone | 5.00 |
| Polysorbate 80 | 15.00 |
| Dextrose | 5.50 |
| Sodium chloride | 4.00 |
| Sodium lauryl sulfate | 1.56 |
| Lecithin | 1.00 |
| L-Cystine | 0.70 |
| Sodium sulfite | 0.20 |
Quality control
Physical/chemical control
| Color | Yellowish-brown |
|---|---|
| pH | 7.0 ± 0.2 at 25 °C |
Microbiological control
Prepare tubes - inoculate: practical range 100 ± 20 CFU, min. 50 CFU (productivity).
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Analytical methodology according to ISO 11133:2014/Amd 1:2018; A2:2020.
Aerobiosis. Incubation at 30-35 °C. Reading at 18-72 h.
| Microorganism | Growth |
|---|---|
| Stph. epidermidisATCC 12228, WDCM 00036 | Good |
| Bacillus subtilisATCC 6633, WDCM 00003 | Good |
| Salmonella typhimuriumATCC 14028, WDCM 00031 | Good |
| Escherichia coliATCC 8739, WDCM 00012 | Good |
| Candida albicansATCC 10231, WDCM 00054 | Good |
| Aspergillus brasiliensisATCC 16404 | Good |
| Ps. aeruginosaATCC 9027, WDCM 00026 | Good |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Good |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- GUISNO, R., I.W. GIBBY & M.J. FOTER (1946) A neutralizing medium for evaluation of the germicidal potency of the quaternary ammonium salts. Amer. J. Pharm. 118:320-323.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 16212 Standard (2017) Cosmetics - Microbiology - Enumeration of yeast and mould.
- ISO 18415 Standard (2017) Cosmetics - Microbiology - Detection of specified and non-specified microorganisms.
- ISO 18416 Standard (2015) Cosmetics - Microbiology - Detection of Candida albicans.
- ISO 21149 Standard (2017) Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria.
- ISO 21149 (2017)/Amd 1:2021 Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria.
- ISO 21150 Standard (2015) Cosmetics - Microbiology - Detection of Escherichia coli.
- ISO 22717 Standard (2015) Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa.
- ISO 22718 Standard (2015). Cosmetics - Microbiology - Detection of Staphylococcus aureus.
- WILLIAMSON, P. & A.M. KLIGMAN (1965) A new method for the quantitative investigation of cutaneous bacteria. J. Inv. Dermatol. 45:498-503.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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