Ready-to-use media
Fluid D EP/USP
For use as rinsing and/or diluting fluid for membrane filtration methods according to USP and harmonized European Pharmacopoeia.
- Cat. No.
- LC-5085
- Category
- Ready-to-use media
- Formats
- 3 formats
- ISO 17025 quality control
- Yes
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 4 months
- Standards
- European PharmacopoeiaUSP
Diluent and non-selective pre-enrichment medium, that has the property of revitalization of samples by the presence of meat peptone in the formulation.
Key features
Applications
Diluent (general use).
Industry
Pharmaceutical/veterinary.
Standards
European Pharmacopoeia, USP.
Description and technique
Inoculate according to final purpose, samples and validated methods.
Presentation
| Code | Presentation | Filling | Packaging |
|---|---|---|---|
| LC-5085 | 10 prepared bottles, 125 mL | 100 ± 3 mL | 1 box with 10 bottles 125 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
| LC-5086 | 10 prepared bottles, 500 mL | 300 ± 5 mL | 1 box with 10 bottles 500 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
| LC-5087 | 6 prepared bottles, 1 L | 850 ± 10 mL | 1 box with 6 bottles 1 L. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
Composition
| Ingredient | g/L |
|---|---|
| Meat peptone | 1.0 |
| Polysorbate 80 | 1.0 |
Quality control
Physical/chemical control
| Color | Colourless |
|---|---|
| pH | 7.1 ± 0.2 at 25 °C; LC-5086: 7.0 ± 0.2 at 25 °C |
Microbiological control
Prepare tubes - inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity).
Subculture in appropriate culture media after T0 (inoculum), 45 min - 1 h at 20-25 °C.
Aerobiosis. Incubation at 35 ± 2 °C, reading after 18-24 hours.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 25922, WDCM 00013 | Good. Recovery ± 30% T0 (original enumeration) |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Good. Recovery ± 30% T0 (original enumeration) |
| Bacillus subtilisATCC 6633, WDCM 00003 | Good. Recovery ± 30% T0 (original enumeration) |
| Candida albicansATCC 10231, WDCM 00054 | Good. Recovery ± 30% T0 (original enumeration) |
| Aspergillus brasiliensisATCC 16404 | Good. Recovery ± 30% T0 (original enumeration) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- EUROPEAN PHARMACOPOEIA 6.0 (2006) 6th ed. § 2.6.2. Biological test. EDQM. Council of Europe. Strasbourg.
- EUROPEAN PHARMACOPOEIA 8.0 (2014) 8th ed. § 2.6.13. Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. EDQM. Council of Europe. Strasbourg.
- USP 33 - NF 28 (2011) <62> Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. USP Corp. Inc. Rockville. MD. USA.
- USP 33 - NF 28 (2011) <71> Sterility Tests. Harmonised Method. USP Corp. Inc. Rockville. MD. USA.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.