Ready-to-use media
Maximum Recovery Diluent (MRD) ISO
Isotonic diluent for the maximal recovery of stressed microorganisms according to ISO standards.
- Cat. No.
- LC-5093
- Category
- Ready-to-use media
- Format
- 10 x 250 mL flasks
- ISO 17025 quality control
- Yes
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 6 months
- Minimum order
- 15 packs
- Standards
- ISO 16212ISO 21149ISO 21150ISO 22717ISO 22718
This formulation combines the osmotic pressure of the physiological saline solution with the protective action of the peptone to obtain good recovery of stressed microorganisms.
Key features
Applications
Diluent (general use).
Industry
Cosmetics, food.
Standards
ISO 16212, ISO 21149, ISO 21150, ISO 22717, ISO 22718.
Presentation
10 prepared bottles, 250 mL, with 225 ± 3 mL.
Description and technique
Description
The sodium chloride ensures isotonic conditions and the low concentration of peptone does not allow cellular growth in the short period (2-4 hours) of time required for the preparation of the dilution bank of the sample.
Technique
According to the ISO method, the sample is diluted in a ratio 1:10 with the Maximum Recovery Diluent and homogenized by a vortex mixer or Stomacher. After a short period (10-15 minutes) of rest, a 1/10 dilution bank with the same diluent is prepared following standard procedures. Plates are inoculated using the range of different concentrations.
Presentation
| Presentation | 10 prepared bottles, 250 mL |
|---|---|
| Filling | 225 ± 3 mL |
| Packaging | 1 box with 10 bottles 250 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
Composition
| Ingredient | g/L |
|---|---|
| Peptone | 1.00 |
| Sodium chloride | 8.50 |
Quality control
Physical/chemical control
| Color | Colourless |
|---|---|
| pH | 7.0 ± 0.2 at 25 °C |
Microbiological control
Prepare tubes / inoculate ≤ 10³ CFU/tube (productivity) / subculture after holding at 20-25 °C for 45 min to 1 h.
Aerobiosis. Incubation at 30-35 °C. Reading at 24-48 h.
Microbiological control according to ISO 11133:2014/Amd 1:2018.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 8739, WDCM 00012 | Good. Recovery ± 30% T0 (original enumeration) |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Good. Recovery ± 30% T0 (original enumeration) |
| Candida albicansATCC 10231, WDCM 00054 | Good. Recovery ± 30% T0 (original enumeration) |
| Ps. aeruginosaATCC 9027, WDCM 00026 | Good. Recovery ± 30% T0 (original enumeration) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ISO 6887-1:1999 Microbiology of food and animal feeding stuffs. Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions - Part 2 (2003): Specific rules for the preparation of meat and meat products.
- ISO 8261:2001 Standard. Milk and milk products - General guidance for the preparation of test samples, initial suspension and decimal dilution for microbiological examination.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 16212 Standard (2017) Cosmetics - Microbiology - Enumeration of yeast and mould.
- ISO 21149 Standard (2017) Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria.
- ISO 21150 Standard (2015) Cosmetics - Microbiology - Detection of Escherichia coli.
- ISO 22717 Standard (2015) Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa.
- ISO 22718 Standard (2015). Cosmetics - Microbiology - Detection of Staphylococcus aureus.
- UNE-EN ISO 11133 (2014). Microbiología de los alimentos para consumo humano, alimentación animal y agua - Preparación, producción, conservación y ensayos de rendimiento de los medios de cultivo.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
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