Ready-to-use media
Sbouraud Dextrose Agar with Chloramphenicol EP/USP/ISO
Medium for the enumeration and cultivation of fungi (mould and yeast).
- Cat. No.
- LC-5072
- Category
- Ready-to-use media
- Format
- 10 x 200 mL flasks
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 4 months
- Standards
- European PharmacopoeiaISO 16212USP
This culture medium differs from the classical Sabouraud Agar only by the addition of chloramphenicol. This thermostable antibiotic has a broad antibacterial spectrum which ensures the selective isolation of fungi from highly contaminated samples.
Key features
Applications
Selective enumeration of yeasts and molds.
Industry
Food, general cultivation, pharmaceutical/veterinary.
Standards
European Pharmacopoeia, ISO 16212, USP.
Presentation
10 prepared bottles, 250 mL, with 200 ± 5 mL.
Description and technique
Technique
To use, the contents of the bottle should be poured into plates. The melting of the culture medium should be carried out according to the manufacturer's instructions, either in a water bath or microwave oven. Never apply direct heat to melt a medium. The melting temperatures and times depend on the shape of the container, the volume of medium and the heat source. Before melting any medium, loosen the screw cap of the container to avoid breaking the container. The medium should be melted only once and used. Media with agar should not be melted repeatedly as their characteristics change with each remelting. Overheating should be avoided as much as prolonged heating, especially with regard to media with an acidic or alkaline pH. Once melted, pour the plates using aseptic techniques.
The technique of inoculation is by streaking methodology or by spiral method.
Incubate the plates right side up aerobically at 20-25 °C for up to 5 days.
(Incubation times greater than those mentioned above or different incubation temperatures may be required depending on the sample or the specifications.)
After incubation, enumerate all the colonies that have appeared onto the surface of the agar.
Each laboratory must evaluate the results according to their specifications.
Presentation
| Presentation | 10 prepared bottles, 250 mL |
|---|---|
| Filling | 200 ± 5 mL |
| Packaging | 1 box with 10 bottles 250 mL. Non-injectable cap. |
Composition
| Ingredient | g/L |
|---|---|
| D(+)-Glucose | 40.0 |
| Peptone from casein | 5.00 |
| Meat peptone | 5.00 |
| Agar | 15.0 |
| Chloramphenicol | 0.05 |
Quality control
Physical/chemical control
| Color | Straw-coloured yellow |
|---|---|
| pH | 5.6 ± 0.2 at 25 °C |
Microbiological control
Melting - pour plates - inoculation: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity).
Growth Promotion Test according to harmonized pharmacopoeial monographs and test methods and ISO 11133:2014.
Aerobiosis. Incubation at 22.5 ± 2.5 °C. Reading at 24-72 h for bacteria and 3-5 days for yeasts and moulds.
| Microorganism | Growth |
|---|---|
| Aspergillus brasiliensisATCC 16404, WDCM 00053 | Good (≥ 50%) |
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 50%) |
| S. cerevisiaeATCC 9763, WDCM 00058 | Good (≥ 50%) |
| Escherichia coliATCC 8739, WDCM 00012 | Inhibited |
| Bacillus subtilisATCC 6633, WDCM 00003 | Inhibited |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- AJELLO, L. (1957) Cultural Methods for Human Pathogenic Fungi. J. Chron. Dis. 5:545-551.
- COLIPA (1997) Guidelines on Microbial Quality Management (MQM). Brussels.
- EUROPEAN PHARMACOPOEIA 8.0 (2014) 8th ed. § 2.6.13. Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. EDQM. Council of Europe. Strasbourg.
- GEORGE, L.K., AJELLO, L. & PAPAGEORGE, C. (1954) Use of Cycloheximide in the Selective Isolation of Fungi Pathogenic to Man. J. Lab. Clin. Med, 44 (422-428).
- HANTSCHKE, D. (1968) Mykosen, 11, (769-778).
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 13681 Standard. (1995). Enumeration of Yeasts and Moulds. Colony Count Technique.
- PAGANO, J. LEVIN, J.D. and TREJO, W. (1957-58) Diagnostic Medium for Differentiation of Species of Candida. Antibiotics Annual, 137-143.
- SABOURAUD, R. (1910) Les Teignes. Masson, Paris.
- USP 33 - NF 28 (2011) <62> Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. USP Corp. Inc. Rockville. MD. USA.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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