RNase inhibitorsBovine RNase InhibitorCat. No. DB-1260Formats 3 formats
RNase inhibitors
Ultima H RNase Inhibitor
The most stable and potent RNase inhibitor on the market
- Cat. No.
- DB-1306
- Category
- RNase inhibitors
- Formats
- 3 formats
- Manufacturer
- DIANA Biotechnologies
- Documents
- Technical specification, PDFsign in to downloadTechnical specification, PDF168 KB
Description
Ultima H is a highly engineered RNase inhibitor of human origin with the highest thermal stability and almost absolute oxidation resistance, leading to robust performance across diverse assay environments. It inhibits the activity of common RNases found in human tissues and body fluids, does not interfere with reverse transcriptases, DNA and RNA polymerases, and sets a new benchmark for reliability in RNA protection. Patent pending.
Key features
Exceptional thermostability
The only protein RNase inhibitor active at temperatures above 55 °C, enabling reverse transcription at higher temperatures with increased yield and sensitivity.
Complete oxidation resistance
The only truly oxidatively resistant RNase inhibitor: retains full activity without DTT and even at 2.0 % H2O2.
Excellent storage and assay stability
Stable for at least 2 months at 25 °C with 1 mM DTT and at least one week at 25 °C without DTT; stable at room temperature in usual PCR buffers for months.
Suitable for direct RT-PCR
Even higher activity than human RNase inhibitor in human tissues: protects 100 % of RNA during direct RT-qPCR from human serum and saliva.
Broad buffer and pH compatibility
Remains fully active in alkaline pH (9.0) and broader assay conditions than competitors.
Lyo-ready
Available in both glycerol and glycerol-free (lyo-ready) formulations.
Applications
- One-step RT-qPCR
- Two-step RT-qPCR
- Direct RT-PCR from human samples
- In vitro transcription and translation
- cDNA synthesis at high temperatures (> 50 °C)
- cDNA synthesis for subsequent cloning
- RNA structural and functional studies
- Expression profiling from single cells
- RNA isolation from biological samples
Technical details
| Origin | Engineered human RNase inhibitor, recombinant |
|---|---|
| Concentration | 40 U/µL |
| Recommended working concentration | 0.5 unit per 1 µL reaction (lower may be sufficient, e.g. one-step RT-qPCR) |
| Storage buffer | 20 mM HEPES pH 7.5, 50 mM KCl, 8 mM DTT, 0.5 mM EDTA, 50 % (v/v) glycerol |
| Thermal stability | 100 % residual activity after 5 min at 55 °C |
| Oxidative stability | 100 % residual activity after 20 min in 2.0 % H2O2 without DTT |
| Storage | Store at −18 °C to −25 °C. Stable for 3 years. Stock solution stable for up to 2 months at 25 °C. |
| Formulations | Glycerol; glycerol-free (lyo-ready) on request |
| Larger sizes | Bulk and OEM sizes available on request |
Performance data
Manufacturer information
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.