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Ultima H RNase Inhibitor Lyo Ready
Product photo: Ultima H RNase Inhibitor Lyo Ready

RNase inhibitors

Ultima H RNase Inhibitor Lyo Ready

Glycerol-free formulation of the most stable RNase inhibitor, for lyophilized assays

Cat. No.
DB-1307
Formats
2 formats

Request a quote

Cat. No.FormatPrice
DB-1307-10kU10,000 units
DB-1307-40kU40,000 units

We reply within 24 hours with a quote and availability.

Description

Glycerol-free, lyo-ready formulation of Ultima H RNase Inhibitor, designed for mixes and assays to be lyophilized. Ultima H is a highly engineered RNase inhibitor of human origin with the highest thermal stability and almost absolute oxidation resistance: it inhibits the activity of common RNases found in human tissues and body fluids and does not interfere with reverse transcriptases, DNA and RNA polymerases.

Key features

Glycerol-free, lyo-ready

Formulated without glycerol for lyophilized RT-PCR and RT-qPCR mixes and assays.

Exceptional thermostability

The only protein RNase inhibitor active at temperatures above 55 °C, enabling reverse transcription at higher temperatures with increased yield and sensitivity.

Complete oxidation resistance

The only truly oxidatively resistant RNase inhibitor: retains full activity without DTT and even at 2.0 % H2O2.

Suitable for direct RT-PCR

Protects 100 % of RNA during direct RT-qPCR from human serum and saliva.

Broad buffer and pH compatibility

Remains fully active in alkaline pH (9.0) and broader assay conditions than competitors.

Applications

  • Lyophilized RT-PCR and RT-qPCR mixes and assays
  • One-step RT-qPCR
  • Two-step RT-qPCR
  • Direct RT-PCR from human samples
  • In vitro transcription and translation
  • RNA-seq library preparation

Technical details

OriginEngineered human RNase inhibitor, recombinant
FormulationGlycerol-free (lyo-ready)
Thermal stability100 % residual activity after 5 min at 55 °C
Oxidative stability100 % residual activity after 20 min in 2.0 % H2O2 without DTT
Inhibited RNasesHuman RNases 1 to 8 and RNase T2; low levels of E. coli RNase I
Larger sizesBulk and OEM sizes available on request

Performance data

Residual inhibitor activity after 20 min pre-incubation with hydrogen peroxide (no DTT): Ultima H retains 100 % activity at 2.0 % H2O2, all competitors drop below 1 %.
Residual inhibitor activity after 20 min pre-incubation with hydrogen peroxide (no DTT): Ultima H retains 100 % activity at 2.0 % H2O2, all competitors drop below 1 %.
Residual inhibitor activity after 5 min at 25, 50, 52.5 and 55 °C: Ultima H is the only RNase inhibitor fully active above 55 °C.
Residual inhibitor activity after 5 min at 25, 50, 52.5 and 55 °C: Ultima H is the only RNase inhibitor fully active above 55 °C.

What you receive

The product

Shipped under controlled temperature when the product requires it.

Lot certificate

Every lot comes with its certificate.

Tracked shipment

Follow your shipment live, from our warehouse to your lab.

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Frequently asked questions

Can I ask for formats other than those listed?

Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.

How do I see the prices?

Prices are shown after you sign in to the customer area, with your account discount already applied.

How long does delivery take?

We give you the availability with the quote, within 24 hours of your request.