Molecular biology media
2xYT Agar
Medium for fibrous bacteriophages.
- Cat. No.
- LC-1167
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
2xYT Agar is a nutritionally rich growth medium recommended for the optimal growth of recombinant strains of E. coli on agar plates. This medium is also used for propagation of M13 bacteriophage or other fibrous bacteriophages for sequencing and phage display.
Key features
Applications
Preparation and recovery of Escherichia coli competent cells.
Industry
Culture media for molecular biology.
Preparation
46 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Bacteriophages are viruses that can only infect and replicate within bacteria. In many cases, these are very specific relationships. Somatic coliphages specifically infect Escherichia coli and in water indicate contamination by human or animal faeces or by wastewater containing such material.
Tryptone and yeast extract are the sources for carbon, nitrogen, vitamins, minerals, and amino acids essential for growth, as well as growth factors that allow phages to reproduce without weakening the host cells. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Many supplements, including antibiotics, are heat-sensitive and cannot be autoclaved. These should be filter-sterilized and added to the medium after it has cooled down and prior to solidification. Escherichia coli grows faster on the enrichment medium as it provides amino acids, nucleotide precursors, vitamins and other metabolites which would otherwise have to be synthesized by the cell.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 46 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 15 |
| Sodium chloride | 5 |
| Tryptone | 16 |
| Yeast extract | 10 |
Preparation and use
Preparation
Suspend 46 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C, mix well and dispense into plates.
Instructions for use
Inoculate and incubate at a temperature of 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Escherichia coliATCC 23724 | Good growth |
| Escherichia coliATCC 33694 | Good growth |
| Escherichia coliATCC 33849 | Good growth |
| Escherichia coliATCC 39403 | Good growth |
| Escherichia coliATCC 47014 | Good growth |
Bibliography
- Principles of Virology: Molecular Biology, Pathogenesis and Control of Animal Viruses. J.S. Flint, L.W. Enquist, V. Racaniello and A.M. Shalka. 2004. 2nd ed. ASM Press, Washington, D.C.
- Principles of Virology: Molecular Biology, Pathogenesis and Control. J.S. Flint, L.W. Enquist, R.M. Krug, V. Racaniello and A.M. Shalka. 2000. 1st ed. Blackwell Science Ltd.
- Flint, et al. (2003) In Principles of Virology: Molecular Biology, Pathogenesis, and Control of Animal Viruses. 2nd ed. ASM Press, Washington DC.
- Sambrook and Russell (2006) In The condensed protocols from Molecular cloning: a laboratory manual, 1st ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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