Molecular biology media
sLB Broth (Buffered)
Medium designed to increase bacterial growth and lead to high yields of low copy plasmids and extra high yields of high copy plasmids.
- Cat. No.
- LC-1199
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
sLB Broth (Buffered) has been formulated to significantly increase cellular density when compared to the traditional LB Broth. In the standard LB Broth, E. coli cells reach an abrupt stationary phase upon consumption of nutrients contained in the medium. Cell multiplication is stopped, some cells die and plasmids are lost.
Key features
Applications
Preparation and recovery of Escherichia coli competent cells.
Industry
Culture media for molecular biology.
Preparation
54.48 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Based on the findings of extensive research, a new formulation has been developed using a proprietary peptone mixture, yeast extract and salts, which allows recombinant E. coli cells to have a higher growth. At the end of the log phase replication continues, thus obtaining higher plasmid DNA yields.
sLB Broth cultures have shown cell stability up to 3 days without cell death, this being a more convenient medium that eliminates the need for constant attention. E. coli growth is higher in sLB Broth and sLB Broth (Buffered) than in standard LB after 3 days at 37 °C.
The special peptone mixture, yeast extract, agar and salts supply essential growth factors such as nitrogen, carbon, sulfur, minerals and vitamins, particularly of the B group. Sodium chloride supplies essential electrolytes: sodium ions for transport and osmotic balance.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 54.48 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Yeast extract, special peptone mixture, salts | 54.48 |
Preparation and use
Preparation
Suspend 54.48 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense in tubes and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Carry out the experimental procedure according to appropriate use or purpose.
- Inoculate and incubate at a temperature of 35 ± 2 °C for 24, 48 and 72 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 24, 48, 72 h.
| Microorganism | Specification |
|---|---|
| Escherichia coli DH5 alpha + pUC19 | Good growth |
Bibliography
- Ausubel, Brent, Kingston, Moore, Seidman, Smith and Struhl (ed.). 1994. Current protocols in molecular biology, vol. 1. Greene Publishing Associates, Inc., Brooklyn, N.Y.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
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