sLB Agar

Based on the findings of extensive research, a new formulation has been developed using a proprietary peptone mixture, yeast extract and salts, which allows recombinant E. coli cells to have a higher growth. At the end of the log phase replication continues, thus obtaining higher plasmid DNA yields.

sLB Agar cultures have shown cell stability up to 3 days without cell death, this being a more convenient medium that eliminates the need for constant attention. E. coli growth is higher in sLB media than in standard LB after 3 days at 37 °C.

The special peptone mixture, yeast extract, agar and salts supply essential growth factors such as nitrogen, carbon, sulfur, minerals and vitamins, particularly of the B group. Sodium chloride supplies essential electrolytes like sodium ions for transport and osmotic balance. Bacteriological agar is the solidifying agent.

Preparation

Suspend 55 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C, mix well and dispense into plates.

Instructions for use

1. Inoculate a 15 mL tube of sLB Broth (LC-1163) with an E. coli sample.
2. Incubate at 37 °C for 24 hours in anaerobic conditions.
3. Take 10 µL of an aliquot of 10⁴ cells/mL and inoculate plates of sLB Agar using a Drigalski spreader.
4. Incubate at 37 °C overnight.

• Ausubel, Brent, Kingston, Moore, Seidman, Smith and Struhl (ed.). 1994. Current protocols in molecular biology, vol. 1. Greene Publishing Associates, Inc., Brooklyn, N.Y.