Molecular biology media
YP Base Medium
For maintaining and developing yeast in molecular biology procedures.
- Cat. No.
- LC-1511
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
YP Base Medium is used for the maintenance and the development of yeast in molecular biology procedures.
Key features
Applications
Growth of yeasts.
Industry
Culture media for molecular biology.
Preparation
30 g in 900 mL of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes, add 100 mL of sterile dextrose at 20%.
Principles and uses
YP Base Medium is also used to cultivate Saccharomyces cerevisiae and other yeasts. Yeasts grow well on a medium containing only a minimal amount of glucose and salts. This medium contains glucose (with the addition of dextrose after autoclaving), salts and proteins, which favors the growth of Saccharomyces cerevisiae and reduces growing times. Yeast extract is the source of vitamins, particularly the B-group, essential for bacterial growth. Peptone provides nitrogen, vitamins, minerals and amino acids.
Saccharomyces cerevisiae has a genome of 14 Mb containing 6000 genes arranged in 16 chromosomes, which have been completely sequenced, and thus, is a species type in microbiology and genetics studies.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 6.5 ± 0.2 |
| Amount | 30 g in 900 mL |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Peptone | 20 |
| Yeast extract | 10 |
Preparation and use
Preparation
Suspend 30 grams of the medium in 900 mL of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 °C for 15 minutes. Cool to room temperature and aseptically add 100 mL of sterile dextrose at 20%. Mix well and dispense into appropriate containers.
Instructions for use
Inoculate and incubate at a temperature of 25 ± 2 °C for 42-48 hours.
Quality control
Microbiological test
Incubation conditions: 25 ± 2 °C / 42-48 h.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Saccharomyces cerevisiaeATCC 9763 | Good growth |
Bibliography
- Guide to yeast genetics and molecular biology. (1991) Ed. Christine Guthrie & Gerald Fink. Methods in Enzymology vol. 194.
- Current protocols in Molecular Biology. Eds. Ausubel, F. M. Brent, R., Kingston, R, E., Moore, D. D., Seidman, J. G., Smith J. A., and Struhl, K. 13,01.-13.2.10.
- The Yeast Genome Directory (1997, May 29) Nature Supp. to volume 387.
- Joseph Sambrook, David W. Russell. The condensed protocols from molecular cloning: a laboratory manual.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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