Molecular biology media
2xYT Medium
For the cultivation of recombinant strains of E. coli and for growth of filamentous bacteriophages.
- Cat. No.
- LC-1507
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
2xYT Medium is a nutritive medium optimized for the growth and maintenance of M13 phages and other filamentous bacteriophages. It is also suitable for growth of recombinant strains of E. coli.
Key features
Applications
Preparation and recovery of competent cells and selective enrichment of Escherichia coli.
Industry
Culture media for molecular biology.
Preparation
31 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Tryptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly the B-group. Sodium chloride supplies essential electrolytes for transport and osmotic balance. The components of the 2xYT Medium include nitrogen and other growth factors that allow bacteriophages to reproduce in large quantities without weakening the host. E. coli grows faster in this enriched medium, as it contains amino acids, precursors of nucleotides, vitamins and other metabolites which otherwise the cell itself would have to synthesize.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 31 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Sodium chloride | 5 |
| Tryptone | 16 |
| Yeast extract | 10 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 31 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Carry out the experimental procedure according to appropriate use or purpose.
- Inoculate and incubate at a temperature of 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Escherichia coliATCC 23724 | Good growth |
| Escherichia coliATCC 33694 | Good growth |
| Escherichia coliATCC 33849 | Good growth |
| Escherichia coliATCC 39403 | Good growth |
| Escherichia coliATCC 47014 | Good growth |
Bibliography
- Principles of Virology: Molecular Biology, Pathogenesis and Control of Animal Viruses. J.S. Flint, L.W. Enquist, V. Racaniello and A.M. Shalka. 2004. 2nd ed. ASM Press, Washington, D.C.
- Joseph Sambrook, David W. Russell. The condensed protocols from molecular cloning: a laboratory manual.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.