Molecular biology media
SOB Medium
For the cultivation of recombinant strains of E. coli.
- Cat. No.
- LC-1541
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
SOB Medium is a nutrient rich medium for the preparation and transformation of competent cells. The transformation requires perforation of the bacteria to allow the introduction of alien DNA inside the cell. In order to survive this process the competent cells need an isotonic rich medium.
Key features
Applications
Preparation and recovery of Escherichia coli competent cells.
Industry
Culture media for molecular biology.
Preparation
26.6 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly the B-group. Sodium chloride and potassium chloride supply essential electrolytes for transport and osmotic balance. Magnesium sulfate is a source of magnesium ions.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 26.6 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Magnesium chloride anhydrous | 0.96 |
| Potassium chloride | 0.186 |
| Sodium chloride | 0.5 |
| Tryptone | 20 |
| Yeast extract | 5 |
Preparation and use
Preparation
Suspend 26.6 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
Inoculate with the transformed cells and incubate at 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Escherichia coliATCC 23724 | Good growth |
| Escherichia coliATCC 33694 | Good growth |
| Escherichia coliATCC 33849 | Good growth |
| Escherichia coliATCC 39403 | Good growth |
| Escherichia coliATCC 47014 | Good growth |
Bibliography
- Joseph Sambrook, David W. Russell. The condensed protocols from molecular cloning: a laboratory manual.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
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