Molecular biology media
Modified Terrific Broth
Medium for use with glycerol in the culture of recombinant E. coli strains.
- Cat. No.
- LC-1548
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Modified Terrific Broth is a medium that supports a high cell density and, when formulated for optimum growth of E. coli, maintains growth in the logarithmic phase for a long time. As a result, it yields a greater number of recombinant proteins and plasmid DNA. In some circumstances it substitutes LB Broth (Lennox) (LC-1231) used in genetic studies.
Key features
Applications
Preparation and recovery of Escherichia coli competent cells.
Industry
Culture media for molecular biology.
Preparation
47.6 g in 900 mL of distilled water; add 4 mL of glycerol and adjust to 1000 mL, boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Tryptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly the B-group. Potassium phosphates act as a buffer system to prevent cell death.
Glycerol is the source of carbohydrates and carbon since it is not fermented to acetic acid as glucose and does not lead to confusing results.
The formulation of this medium was modified from the Terrific Broth (LC-1246) with a different concentration of biological buffer.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 7.2 ± 0.2 |
| Amount | 47.6 g in 900 mL |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dipotassium phosphate | 9.4 |
| Monopotassium phosphate | 2.2 |
| Tryptone | 12 |
| Yeast extract | 24 |
Preparation and use
Preparation
Suspend 47.6 grams of the medium in 900 mL of distilled water. Mix well. Add 4 mL of glycerol, adjust to a final volume of 1000 mL, and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Carry out the experimental procedure according to appropriate use or purpose.
- Inoculate and incubate at a temperature of 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Escherichia coliATCC 23724 | Good growth |
| Escherichia coliATCC 33694 | Good growth |
| Escherichia coliATCC 33849 | Good growth |
| Escherichia coliATCC 39403 | Good growth |
| Escherichia coliATCC 47014 | Good growth |
Bibliography
- SAMBROC, J., E.F. FRITSCH, T. MANIATIS (1989) MOLECULAR CLONING.
What you receive
The product
Shipped under controlled temperature when the product requires it.
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