Molecular biology media
YPD Broth
For maintaining and developing yeasts in molecular biology procedures.
- Cat. No.
- LC-1547
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
YPD Broth is used for the maintenance and the development of yeast in molecular biology procedures. The formula is the same as in YP Base Medium (LC-1511) but with the dextrose added.
Key features
Applications
Enrichment of yeasts and protein expression in yeasts.
Industry
Culture media for molecular biology.
Preparation
50 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 118 °C for 15 minutes.
Principles and uses
YPD Broth is also used to cultivate Saccharomyces cerevisiae and other yeasts. Yeasts grow well on a medium containing only a minimal amount of glucose and salts. This medium contains dextrose, salts and proteins, which favors the growth of Saccharomyces cerevisiae and reduces growing times. Yeast extract is the source of vitamins, particularly the B-group, essential for bacterial growth. Peptone provides nitrogen, vitamins, minerals and amino acids.
Saccharomyces cerevisiae has a genome of 14 Mb containing 6000 genes arranged in 16 chromosomes, which have been completely sequenced, and thus, is a species type in microbiology and genetics studies.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 6.5 ± 0.2 |
| Amount per litre | 50 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 20 |
| Peptone | 20 |
| Yeast extract | 10 |
Preparation and use
Preparation
Suspend 50 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 118 °C for 15 minutes. Avoid overheating.
Instructions for use
- Carry out the experimental procedure according to appropriate use or purpose.
- Inoculate and incubate at a temperature of 25 ± 2 °C for 42-48 hours.
Quality control
Microbiological test
Incubation conditions: 25 ± 2 °C / 42-48 h.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Saccharomyces cerevisiaeATCC 9080 | Good growth |
| Saccharomyces cerevisiaeATCC 9763 | Good growth |
Bibliography
- Guide to yeast genetics and molecular biology. (1991) Ed. Christine Guthrie & Gerald Fink. Methods in Enzymology vol. 194.
- Current protocols in Molecular Biology. Eds. Ausubel, F. M. Brent, R., Kingston, R, E., Moore, D. D., Seidman, J. G., Smith J. A., and Struhl, K. 13,01.-13.2.10.
- The Yeast Genome Directory (1997, May 29) Nature Supp. to volume 387.
- Joseph Sambrook, David W. Russell. The condensed protocols from molecular cloning: a laboratory manual.
What you receive
The product
Shipped under controlled temperature when the product requires it.
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