Molecular biology media
YPD Agar
For maintaining and developing yeast in molecular biology procedures.
- Cat. No.
- LC-1546
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
YPD Agar is used for maintaining and developing yeast in molecular microbiology procedures. The formula is the same as in YP Agar Base Medium (LC-1513) but with the dextrose added.
Key features
Applications
Enrichment of yeasts.
Industry
Culture media for molecular biology.
Preparation
65 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
YPD Agar is also used to cultivate Saccharomyces cerevisiae and other yeasts. Yeasts grow well on a medium containing only a minimal amount of glucose and salts. This medium contains dextrose (with the addition of dextrose after autoclaving), salts and proteins, which favors the growth of Saccharomyces cerevisiae and reduces growing times. Yeast extract is the source of vitamins, particularly the B-group. Peptone provides nitrogen, vitamins, minerals and amino acids. Bacteriological agar is the solidifying agent.
Saccharomyces cerevisiae has a genome of 14 Mb containing 6000 genes arranged in 16 chromosomes, which have been completely sequenced, and thus, is a species type in microbiology and genetics studies.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 6.5 ± 0.2 |
| Amount per litre | 65 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 15 |
| Dextrose | 20 |
| Peptone | 20 |
| Yeast extract | 10 |
Preparation and use
Preparation
Suspend 65 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C, mix well and dispense into plates.
Instructions for use
- This medium can be inoculated directly or after the enrichment broth YPD Broth (LC-1547).
- Spread the plates of YPD Agar and incubate at 25 ± 2 °C for 42-48 hours.
Quality control
Microbiological test
Incubation conditions: 25 ± 2 °C / 42-48 h.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Saccharomyces cerevisiaeATCC 9080 | Good growth |
Bibliography
- Guide to yeast genetics and molecular biology. (1991) Ed. Christine Guthrie & Gerald Fink. Methods in Enzymology vol. 194.
- Current protocols in Molecular Biology. Eds. Ausubel, F. M. Brent, R., Kingston, R, E., Moore, D. D., Seidman, J. G., Smith J. A., and Struhl, K. 13,01.-13.2.10.
- The Yeast Genome Directory (1997, May 29) Nature Supp. to volume 387.
- The condensed protocols from molecular cloning: a laboratory manual / Joseph Sambrook, David W. Russell.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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