Molecular biology media
RM Base Medium
Solid medium for the maintenance and propagation of the promoter PL in the E. coli strains GI724, GI826 and GI698.
- Cat. No.
- LC-1538
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
RM Base Medium is used for the maintenance and propagation of the promoter PL in E. coli strains GI724, GI826 and GI698 and to increase the yield of plasmid for sequencing positive clones. These strains have the gene Lambda cI repressor, under the control of the promoter tryptophane inducible, trp. This medium has low tryptophane levels.
Key features
Applications
Preparation and recovery of Escherichia coli competent cells.
Industry
Culture media for molecular biology.
Preparation
30.6 g in 900 mL of distilled water; add 20 mL of 50% glycerol, sterilize in autoclave at 121 °C for 15 minutes, add ampicillin.
Principles and uses
Casaminoacids provide the necessary nutrients and cofactors required for excellent growth of recombinant strains of E. coli. Due to its higher degree of digestion, casaminoacids are an excellent source of free amino acids. Phosphates act as a buffer system. Ammonium chloride and magnesium sulfate provide essential ions. Sodium chloride supplies essential electrolytes for transport and osmotic balance. To promote growth it may require the addition of glucose.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount | 30.6 g in 900 mL |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Ammonium chloride | 1 |
| Casaminoacids | 20 |
| Disodium phosphate | 6 |
| Magnesium citrate | 0.095 |
| Sodium chloride | 0.5 |
| Monosodium phosphate | 3 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 30.6 grams of the dehydrated medium in 900 mL of distilled water, add 20 mL of 50% glycerol and adjust to a final volume of 1000 mL. Mix well. Heat with frequent agitation until complete dissolution. Distribute in appropriate containers and sterilize in the autoclave at 121 °C for 15 minutes. Add 1 mL/liter of 100 µg/mL of ampicillin under sterile conditions and mix well.
Instructions for use
Inoculate and incubate at a temperature of 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Escherichia coliATCC 23724 | Good growth |
| Escherichia coliATCC 33694 | Good growth |
| Escherichia coliATCC 33849 | Good growth |
| Escherichia coliATCC 39403 | Good growth |
| Escherichia coliATCC 47014 | Good growth |
Bibliography
- La Vallie, E, R. et al. (1992) Bio/Technology 11: 187-193.
- Mieschendahl, M. et al. (1996) Bio/Technology 4: 802-808.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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