Molecular biology media
Yeast Nitrogen Base w/o Amino Acids
For yeast classification based on amino acids and carbohydrates requirements.
- Cat. No.
- LC-1545
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Yeast Nitrogen Base w/o Amino Acids is used for classifying yeasts based on carbon and nitrogen requirements and is prepared according to the formulas of Wickerharm and Burkholder. The medium contains all the essential vitamins and inorganic salts needed to cultivate yeasts, except for the amino acids and carbohydrate sources. Ammonium sulphate is included as a readily available nitrogen source for nitrogen assimilation.
Key features
Applications
Differentiation of yeasts.
Industry
Culture media for molecular biology, food.
Preparation
10X solution: 6.7 g in 100 mL of distilled water with 5 g of dextrose and 5-10 mg of the desired amino acid; do not boil, do not autoclave, sterilize by filtration.
Principles and uses
This medium is used in many applications for the study of yeast in molecular biology as it is useful for the determination of amino acids and carbohydrate utilization.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Off-white |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 5.4 ± 0.2 |
| Amount | 6.7 g in 100 mL (10X solution) |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Ammonium sulfate | 5 |
| Boric acid | 0.0005 |
| Calcium chloride | 0.1 |
| Calcium pantothenate | 0.0004 |
| Ferric chloride | 0.0002 |
| Inositol | 0.002 |
| Magnesium sulfate | 0.5 |
| Manganese sulfate | 0.0004 |
| Monopotassium phosphate | 1 |
| Niacin | 0.0004 |
| p-Aminobenzoic acid | 0.0002 |
| Potassium iodide | 0.0001 |
| Pyridoxine hydrochloride | 0.0004 |
| Riboflavin | 0.0002 |
| Sodium chloride | 0.1 |
| Sodium molybdate | 0.0002 |
| Thiamine hydrochloride | 0.0004 |
| Zinc sulfate | 0.0004 |
| Copper sulfate | 0.04 mg |
| Folic acid | 0.002 mg |
| Biotin | 0.002 mg |
Preparation and use
Preparation
Prepare a 10X solution by dissolving 6.7 grams of the medium in 100 mL of distilled water with 5 grams of dextrose, or the equivalent amount of another carbohydrate, and 5-10 mg of the desired amino acid. Mix well. Heat with frequent agitation until complete dissolution. DO NOT BOIL. DO NOT AUTOCLAVE. Sterilize the solution by filtration. Prepare the final medium by aseptically pipetting 0.5 mL of the 10X solution to 4.5 mL of distilled water. Swirl to mix solution before inoculation.
Instructions for use
- Inoculate the prepared tubed medium very lightly with the test organism.
- Incubate at 25-30 °C for 2-5 days.
- After incubation, shake the tubes to suspend growth.
- Read for growth.
- Carry out the carbon and nitrogen assimilation tests described in reference manuals.
Quality control
Microbiological test
Incubation conditions: 25-30 °C / 2-5 days.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Hanseniaspora uvarumATCC 32856 | Good growth |
| Saccharomyces cerevisiaeATCC 9080 | Good growth |
Bibliography
- Shadomy, S., and Espinel Ingroff, A. 1980. Susceptibility Testing with Antifungal Drugs, p. 647-653. In E. H. Lennete, A. Balows, W. J. Hausler, Jr., and J.P. Truant, Manual of Clinical Microbiology, 3rd Ed., American Society for Microbiology, Washington, D.C.
- U.S. Dept. Agric. Tech. Bull. No. 1029, 1951.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.