Molecular biology media
NZCYM Broth
For the cultivation of recombinant E. coli strains.
- Cat. No.
- LC-1549
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
NZCYM Broth is used as an enrichment medium for the cultivation of recombinant strains of E. coli and propagating lambda bacteriophages developed by Blattner and colleagues.
Key features
Applications
Preparation and recovery of Escherichia coli competent cells.
Industry
Culture media for molecular biology.
Preparation
22 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
E. coli grows fast in this enrichment medium, which supplies the amino acids, vitamins and other metabolites as nucleotide precursors and other factors that otherwise would be synthesized by the cell. Casein digest, yeast extract and casamino acids provide the necessary nutrients and cofactors required for excellent growth of recombinant strains of E. coli. Magnesium sulfate is the source of magnesium ions required in a wide variety of enzymatic reactions, including DNA replication.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 22 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Casaminoacids | 1 |
| Magnesium sulfate | 0.98 |
| Pancreatic digest of casein | 10 |
| Sodium chloride | 5 |
| Yeast extract | 5 |
Preparation and use
Preparation
Suspend 22 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Carry out the experimental procedure according to appropriate use or purpose.
- Inoculate and incubate at a temperature of 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Escherichia coliATCC 23724 | Good growth |
| Escherichia coliATCC 33694 | Good growth |
| Escherichia coliATCC 33849 | Good growth |
| Escherichia coliATCC 39403 | Good growth |
| Escherichia coliATCC 47014 | Good growth |
Bibliography
- Blattner, F. R., B. G. Williams, A. E. Blechl, K. Denniston-Thompson, H. E. Faber, L. A. Furlong, D. J. Grunwald, D. O. Kiefer, D. D. Moore, J. W. Schumm, E. L. Sheldon, and O. Smithies. 1977. Charon phages: Safer derivatives of bacteriophage Lambda for DNA cloning. Science 196:161.
- The condensed protocols from molecular cloning: a laboratory manual / Joseph Sambrook, David W. Russell.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
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