Molecular biology media
Yeast Nitrogen Base w/o Added Aminoacids and w/o Ammonium Sulfate
For yeast classification based on carbon and nitrogen requirements.
- Cat. No.
- LC-1553
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Yeast Nitrogen Base w/o Added Aminoacids and w/o Ammonium Sulfate is used for classifying yeasts based on carbon and nitrogen requirements and is prepared according to the formulas of Wickerharm and Burkholder. The medium contains all the essential vitamins and inorganic salts needed to cultivate yeasts, except for the amino acids and carbohydrate sources.
Key features
Applications
Differentiation of yeasts.
Industry
Culture media for molecular biology.
Preparation
10X solution: 1.7 g in 100 mL of distilled water with 5 g of dextrose and 5-10 mg of the desired amino acid; do not boil, do not autoclave, sterilize by filtration.
Principles and uses
This medium is used in many applications for the study of yeast in molecular biology as it is useful for the determination of amino acids and carbohydrate utilization.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Off-white |
| Color of the prepared medium | Colorless |
| Final pH (25 °C) | 4.5 ± 0.2 |
| Amount | 1.7 g in 100 mL (10X solution) |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Biotin | 0.000002 |
| Boric acid | 0.0005 |
| Calcium chloride | 0.1 |
| Calcium pantothenate | 0.0004 |
| Ferric chloride | 0.0002 |
| Folic acid | 0.000002 |
| Inositol | 0.002 |
| Magnesium sulfate | 0.5 |
| Manganese sulfate | 0.0004 |
| Monopotassium phosphate | 1 |
| Niacin | 0.0004 |
| p-Aminobenzoic acid | 0.0002 |
| Potassium iodide | 0.0001 |
| Riboflavin | 0.0002 |
| Sodium chloride | 0.1 |
| Sodium molybdate | 0.0002 |
| Thiamine hydrochloride | 0.0004 |
| Zinc sulfate | 0.0004 |
| Cupric sulphate | 0.00004 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Prepare a 10X solution by dissolving 1.7 grams of the medium in 100 mL of distilled water with 5 grams of dextrose, or the equivalent amount of another carbohydrate, and 5-10 mg of the desired amino acid. Mix well. Heat with frequent agitation until complete dissolution. DO NOT BOIL. DO NOT AUTOCLAVE. Sterilize the solution by filtration. Prepare the final medium by aseptically pipetting 0.5 mL of the 10X solution to 4.5 mL of distilled water. Swirl to mix solution before inoculation.
Instructions for use
Inoculate and incubate at a temperature of 25-30 °C for 2-5 days.
Quality control
Microbiological test
Incubation conditions: 25-30 °C / 2-5 days.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Saccharomyces cerevisiaeATCC 9080 | Good growth |
| Saccharomyces cerevisiaeATCC 9763 | Good growth |
| Kloeckera apiculataATCC 9774 | Good growth |
Bibliography
- Shadomy, S., and Espinel Ingroff, A. 1980. Susceptibility Testing with Antifungal Drugs, p. 647-653. In E. H. Lennete, A. Balows, W. J. Hausler, Jr., and J.P. Truant, Manual of Clinical Microbiology, 3rd Ed., American Society for Microbiology, Washington, D.C.
- U.S. Dept. Agric. Tech. Bull. No. 1029, 1951.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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