Molecular biology media
Induction Base Medium
Medium for maintenance and propagation of the PL promoter in E. coli strains GI724, GI826 and GI698.
- Cat. No.
- LC-1540
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Induction Base Medium is used for the maintenance and propagation of the PL promoter in E. coli strains GI724, GI826 and GI698 and to increase plasmid yield during sequencing of positive clones. These strains contain the Lambda cI repressor gene under the control of the tryptophan-inducible trp promoter. This medium has low levels of tryptophan.
Key features
Applications
Preparation and recovery of Escherichia coli competent cells.
Industry
Culture media for molecular biology.
Preparation
12.6 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes, add glucose and ampicillin.
Principles and uses
Casaminoacids provide the necessary nutrients and cofactors for a good growth of recombinant E. coli strains. Due to their greater degree of digestion, casaminoacids are an excellent source of free amino acids. Phosphates act as a buffer system. Ammonium chloride and magnesium sulfate provide essential ions for transport and osmotic balance.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 12.6 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Ammonium chloride | 1 |
| Casaminoacids | 2 |
| Disodium phosphate | 6 |
| Monopotassium phosphate | 3 |
| Sodium chloride | 0.5 |
| Magnesium chloride | 0.095 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 12.6 grams of medium in one liter of distilled water. Dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 °C for 15 minutes. Cool to room temperature, add 25 mL of a 20% glucose solution and 1 mL of ampicillin (100 g/mL) under sterile conditions. Mix well.
Instructions for use
Consult an appropriate reference for recommended test procedures.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Escherichia coliATCC 23724 | Good growth |
| Escherichia coliATCC 33694 | Good growth |
| Escherichia coliATCC 33849 | Good growth |
| Escherichia coliATCC 39403 | Good growth |
| Escherichia coliATCC 47014 | Good growth |
Bibliography
- La Vallie, E, R. et al. (1992) Bio/Technology 11: 187-193.
- Mieschendahl, M. et al. (1996) Bio/Technology 4: 802-808.
What you receive
The product
Shipped under controlled temperature when the product requires it.
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