Molecular biology media
Terrific Broth (Non animal origin)
Medium used with glycerol for the cultivation of recombinant strains of E. coli.
- Cat. No.
- LC-1499
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Terrific Broth (Non animal origin) is as Terrific Broth (LC-1246) but changing the animal origin raw materials to vegetal origin ones. It is a medium which supports a high cell density and, in the case it is formulated for optimum growth of E. coli, maintains growth in the logarithmic phase for a long time. As a result it yields a greater number of recombinant proteins and plasmid DNA. In some circumstances it substitutes LB Broth (Lennox) (LC-1231) used in genetic studies.
Key features
Applications
Protein expression in Escherichia coli.
Industry
Culture media for molecular biology.
Preparation
50.8 g in 1 liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Vegetable origin peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly the B-group. Potassium phosphates act as a buffer system to prevent cell death.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 7.2 ± 0.2 |
| Amount per litre | 50.8 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dipotassium phosphate | 12.5 |
| Monopotassium phosphate | 2.3 |
| Yeast extract | 24 |
| Vegetable peptone | 12 |
Preparation and use
Preparation
Suspend 50.8 grams of the medium in 1 liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
Inoculate and incubate at 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Escherichia coliATCC 23724 | Good growth |
| Escherichia coliATCC 33694 | Good growth |
| Escherichia coliATCC 33849 | Good growth |
| Escherichia coliATCC 39403 | Good growth |
| Escherichia coliATCC 47014 | Good growth |
Bibliography
- Joseph Sambrook, David W. Russell. The condensed protocols from molecular cloning: a laboratory manual.
What you receive
The product
Shipped under controlled temperature when the product requires it.
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