Buffered Peptone Water (Double concentration)

Inoculate according to final purpose, samples and validated methods.

2X concentrated medium. Dilute to 1X so that the diluent composition complies with the specification of the ISO Standard 6579 for the detection of Salmonella in foods and other ISO Standards (6785, 6887, 8261).

If necessary, adjust the final pH.

Each bag is intended for use with an automatic dispenser in laboratories requiring large volumes of broth media or diluent.

Discard any partially used bag to avoid contamination.

The bag has multiple connection points: 1 penetrable cap (injection port) of latex-free polycarbonate, for any additive injection required, and an injection (vial stopper) to connect to any standard laboratory dosing equipment with a connector.

Once completely empty, the bag can be disposed of along with normal plastic (PVC).

(1) Equivalent to 18.0 g of disodium hydrogen phosphate dodecahydrate.

Physical/chemical control

Microbiological control

Dilute to normal concentration.

Prepare tubes / inoculate 100 ± 20 CFU (productivity) / subculture after holding at 20-25 °C for 45 min to 1 h, E. coli & Staphyl.; Listeria 1 h at 20 ± 2 °C.

Microbiological control according to ISO 11133:2014/Amd 1:2018.

Analytical methodology according to ISO 11133:2014/Amd 1:2018; A2:2020.

Aerobiosis. Incubation at 37 ± 1 °C, reading after 18 ± 2 h.

Sterility control

Incubation 48 h at 30-35 °C and 48 h at 20-25 °C: NO GROWTH.

Check at 7 days after incubation in same conditions.

• ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
• ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
• ISO Standard 6579-1 (2017) Microbiology of food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp.
• ISO 6785 (2001) Milk and milk products. Detection of Salmonella spp.
• ISO 6887-1 (1999) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 1: General rules for the preparation of the initial suspension and decimal dilutions.
• ISO 6887-2 (2003) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 2: Specific rules for the preparation of meat and meat products.
• ISO 6887-3 (2003) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 3: Specific rules for the preparation of fish and fishery products.
• ISO 6887-4 (2003) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 4: Specific rules for the preparation of products other than milk and milk products, meat and meat products and fish and fishery products.
• ISO/DIS 6887-5 (2009) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 5: Specific rules for the preparation of milk and milk products.
• ISO 8261 (2001) Milk and milk products. General guidance for the preparation of test samples for microbiological examination.
• ISO 21528-1:2004 Standard. Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 1: Detection and enumeration by MPN technique with pre-enrichment.
• ISO/TS 22964 (2006) Milk and milk products - Detection of Enterobacter sakazakii.
• PASCUAL ANDERSON, Mª R. (1992) Microbiología Alimentaria. Díaz de Santos, S.A. Madrid.
• UNE-EN ISO 11133 (2014). Microbiología de los alimentos para consumo humano, alimentación animal y agua - Preparación, producción, conservación y ensayos de rendimiento de los medios de cultivo.