Ready-to-use media
Maximun Recovery Diluent (MRD)
Isotonic diluent for the maximal recovery of stressed microorganisms according to ISO standards.
- Cat. No.
- LC-6710
- Category
- Ready-to-use media
- Formats
- 2 formats
- ISO 17025 quality control
- Yes
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 6 months
- Standards
- ISO 11133ISO 22718ISO 6887ISO 8199
This formulation combines the osmotic pressure of the physiological saline solution with the protective action of the peptone to obtain good recovery of stressed microorganisms.
Key features
Applications
Diluent (general use).
Industry
General cultivation.
Standards
ISO 11133, ISO 22718, ISO 6887, ISO 8199.
Description and technique
Description
The sodium chloride ensures isotonic conditions and the low concentration of peptone does not allow cellular growth in the short period of time (2-4 hours) required for the preparation of the dilution bank of the sample.
Technique
According to the ISO method, the sample is diluted in a ratio 1:10 with the Maximum Recovery Diluent and homogenized by a vortex mixer or Stomacher. After a short period (10-15 minutes) of rest, a 1/10 dilution bank with the same diluent is prepared following standard procedures. Plates are inoculated using the range of different concentrations.
Inoculate according to final purpose, samples and validated methods.
Each bag is intended for use with an automatic dispenser in laboratories requiring large volumes of broth media or diluent.
Discard any partially used bag to avoid contamination.
The bag has multiple connection points: 1 penetrable cap (injection port) of latex-free polycarbonate, for any additive injection required, and an injection (vial stopper) to connect to any standard laboratory dosing equipment with a connector.
Once completely empty, the bag can be disposed of along with normal plastic (PVC).
Presentation
| Code | Presentation | Filling | Packaging |
|---|---|---|---|
| LC-6710 | 3 prepared bags, 3 L | 3000 ± 10 mL | 1 box with 3 bags of 3 L. PVC plasticizer free sterile bag with 1 vial stopper + 1 penetrable cap. Dimensions: 23 x 32 cm. For use in food testing. |
| LC-6711 | 2 prepared bags, 5 L | 5000 ± 15 mL | 2 boxes, with 1 bag of 5 L per box, joined by a strip. PVC plasticizer free sterile bag with 1 vial stopper + 1 penetrable cap. Dimensions: 27 x 40 cm. For use in food testing. |
Composition
| Ingredient | g/L |
|---|---|
| Peptone | 1.00 |
| Sodium chloride | 8.50 |
Quality control
Physical/chemical control
| Color | Colourless |
|---|---|
| pH | 7 ± 0.2 at 25 °C |
Microbiological control
Prepare tubes / inoculate 10³-10⁴ (productivity) / subculture after holding at 20-25 °C for 45 min to 1 h.
Aerobiosis. Incubation at 35 ± 2 °C, reading at 24-48 hours.
| Microorganism | Growth |
|---|---|
| Candida albicansATCC 10231, WDCM 00054 | Good. Recovery ± 30% T0 (original enumeration) |
| Escherichia coliATCC 8739, WDCM 00012 | Good. Recovery ± 30% T0 (original enumeration) |
| Ps. aeruginosaATCC 9027, WDCM 00026 | Good. Recovery ± 30% T0 (original enumeration) |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Good. Recovery ± 30% T0 (original enumeration) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ISO 6887-1:1999 Microbiology of food and animal feeding stuffs. Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions - Part 2 (2003): Specific rules for the preparation of meat and meat products.
- ISO 8261:2001 Standard. Milk and milk products - General guidance for the preparation of test samples, initial suspension and decimal dilution for microbiological examination.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 16212 Standard (2017) Cosmetics - Microbiology - Enumeration of yeast and mould.
- ISO 21149 Standard (2017) Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria.
- ISO 21150 Standard (2015) Cosmetics - Microbiology - Detection of Escherichia coli.
- ISO 22717 Standard (2015) Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa.
- ISO 22718 Standard (2015). Cosmetics - Microbiology - Detection of Staphylococcus aureus.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.