Ready-to-use media
Chromogenic Cronobacter Isolation Agar (CCI) (ISO)
Solid differential culture medium for the presumptive isolation of Cronobacter colonies, according to ISO 22964:2017.
- Cat. No.
- LC-866
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Minimum order
- 10 packs
- Standards
- ISO 22964
CCI Agar is the only culture medium prescribed for the isolation of presumptive Cronobacter colonies in the horizontal method for the detection of Cronobacter in food, according to ISO 22964:2017.
Key features
Applications
Selective isolation of Cronobacter sakazakii (chromogenic medium).
Industry
Dairy products, food.
Standards
ISO 22964.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Description and technique
Technique
The surface of CCI agar is inoculated from the enrichment tubes to obtain separated colonies. Plates are incubated at 41.5 °C for 24 ± 2 hours. After incubation, the presence of typical colonies of presumptive Cronobacter is observed and recorded.
On this medium the presumptive colonies of Cronobacter are small or medium (1-3 mm Ø) and blue or blue-green. The colonies of other bacteria are usually white, or white with a darker center, green, gray or even black. Some other pigmented colonies may appear yellow, orange or red.
For a more detailed methodology, the technician is referred to the ISO 22964:2017 standard.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Tryptic digest of casein | 7.00 |
| Yeast extract | 3.00 |
| Sodium chloride | 5.00 |
| Sodium desoxycholate | 0.25 |
| 5-Br-4-Cl-3-indolyl-alpha-D-glucopyranoside | 0.15 |
| Ammonium iron(III) citrate | 1.00 |
| Sodium thiosulphate | 1.00 |
| Agar | 15.00 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|---|
| pH | 7.3 ± 0.2 at 25 °C |
Microbiological control
Spiral spreading: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity).
Aerobiosis. Incubation at 41.5 ± 1 °C, reading at 24 ± 3 h.
| Microorganism | Growth |
|---|---|
| Cr. muytjensii (formerly E. sakazakii)ATCC 51329 | Good. Blue-greenish colonies (1-3 mm Ø) |
| Cronobacter sakazakiiATCC 29544, WDCM 00214 | Good. Blue-greenish colonies (1-3 mm Ø) |
| Stph. aureusATCC 25923, WDCM 00034 | Inhibited |
| Enterobacter aerogenesATCC 13048, WDCM 00083 | Good. Colourless colonies |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ISO 22964 Standard (2017) Microbiology of the food chain - Horizontal method for the detection of Cronobacter spp.
- IVERSEN C., A. LEHNER, N. MULLANE, J. MARUGG, S. FANNING, R. STEPHAN, and H. JOOSTEN (2007) The identification of Cronobacter spp. (Enterobacter sakazakii). J. Clin. Microbiol. 45:3814-3816.
- IVERSEN, C., P. DRUGGAN, S. SCHUMACHER, A. LEHNER, C. FEER, K. GSCHWEND, H. JOOSTEN and R. STEPHAN (2008) Development of a Novel Screening Method for the Isolation of "Cronobacter" spp. (Enterobacter sakazakii). Appl. Environm. Microbiol. 74:2550-2553.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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