Ready-to-use media
Sabouraud Chloramphenicol Dextrose Agar. Double Wrapped. Irradiated EP/USP/ISO
Medium for the enumeration and cultivation of fungi (mould and yeast).
- Cat. No.
- LC-843
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Standards
- European PharmacopoeiaISO 16212USP
This culture medium differs from the classical Sabouraud Agar only by the addition of chloramphenicol. This thermostable antibiotic has a broad antibacterial spectrum which ensures the selective isolation of fungi from highly contaminated samples.
Key features
Applications
Selective enumeration of yeasts and molds.
Industry
Environmental monitoring, food, general cultivation, pharmaceutical/veterinary.
Standards
European Pharmacopoeia, ISO 16212, USP.
Presentation
20 irradiated plates, 90 mm, double wrapping, with 21 ± 2 mL.
Description and technique
Description
This medium is also well suited for air environmental sampling (total compatibility with most commercially available air samplers) or for other types of environmental sampling (fingers or gloves of operators, swab streaking).
Technique
The technique of inoculation is by streaking methodology or by spiral method.
Incubate the plates right side up aerobically at 20-25 °C for up to 5 days.
(Incubation times greater than those mentioned above or different incubation temperatures may be required depending on the sample or the specifications.)
After incubation, enumerate all the colonies that have appeared onto the surface of the agar.
Each laboratory must evaluate the results according to their specifications.
Presentation
| Presentation | 20 irradiated plates, 90 mm, double wrapping |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 cellophane bags (double wrapping) with 10 plates/bag. Every pack exhibits an irradiation indicator stuck on the side of the bag (8-14 kGy). |
Composition
| Ingredient | g/L |
|---|---|
| D(+)-Glucose | 40.0 |
| Peptone from casein | 5.00 |
| Meat peptone | 5.00 |
| Agar | 15.0 |
| Chloramphenicol | 0.05 |
Quality control
Physical/chemical control
| Color | Straw-coloured yellow |
|---|---|
| pH | 5.6 ± 0.2 at 25 °C |
Microbiological control
Growth Promotion Test according to harmonized pharmacopoeial monographs and test methods and ISO 11133:2014.
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ (selectivity).
Aerobiosis. Incubation at 20-25 °C, reading after 24-72 hours for bacteria and 3-5 days for yeasts and moulds.
| Microorganism | Growth |
|---|---|
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 50%) |
| Aspergillus brasiliensisATCC 16404, WDCM 00053 | Good (≥ 50%) |
| S. cerevisiaeATCC 9763, WDCM 00058 | Good (≥ 50%) |
| Bacillus subtilisATCC 6633, WDCM 00003 | Inhibited |
| Escherichia coliATCC 8739, WDCM 00012 | Inhibited |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- AJELLO, L. (1957) Cultural Methods for Human Pathogenic Fungi. J. Chron. Dis. 5:545-551.
- EUROPEAN PHARMACOPOEIA 6.0 (2008) 6th ed. § 2.6.13. Microbiological examination of non-sterile products: Test for specified microorganisms. EDQM. Council of Europe. Strasbourg.
- GEORGE, L.K., AJELLO, L. & PAPAGEORGE, C. (1954) Use of Cycloheximide in the Selective Isolation of Fungi Pathogenic to Man. J. Lab. Clin. Med, 44 (422-428).
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 16212 Standard (2017) Cosmetics - Microbiology - Enumeration of yeast and mould.
- PAGANO, J. LEVIN, J.D. & TREJO, W. (1957-58) Diagnostic Medium for Differentiation of Species of Candida. Antibiotics Annual, 137-143.
- SABOURAUD, R. (1910) Les Teignes. Masson, Paris.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.