Ready-to-use media
Sabouraud Chloramphenicol Dextrose Agar Triple Wrapped. Irradiated. EP/USP/ISO
Medium for the enumeration and cultivation of fungi (mould and yeast).
- Cat. No.
- LC-842
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Standards
- European PharmacopoeiaISO 16212USP
This culture medium differs from the classical Sabouraud Agar only by the addition of chloramphenicol. This thermostable antibiotic has a broad antibacterial spectrum which ensures the selective isolation of fungi from highly contaminated samples.
Key features
Applications
Selective enumeration of yeasts and molds.
Industry
Environmental monitoring, food, general cultivation, pharmaceutical/veterinary.
Standards
European Pharmacopoeia, ISO 16212, USP.
Presentation
20 irradiated plates, 90 mm, triple wrapping, with 21 ± 1 mL.
Description and technique
Technique
For plate inoculation, follow the laboratory's standard methods or the applicable norms (spiral plating method, ecometric methods, streak plating, dilution banks, spread plating with Drigalski rod, etc.).
Attention: Petri plates are used for monitoring the microbiological contamination of surface and air inside cleanrooms, isolators, RABS, food industries and hospitals. The double/triple irradiated wrapping ensures that the package itself doesn't contaminate the environment as the first wrapper is removed just before entering the clean area.
Presentation
| Presentation | 20 irradiated plates, 90 mm, triple wrapping |
|---|---|
| Filling | 21 ± 1 mL |
| Packaging | 1 box with 3 cellophane bags (triple wrapping) with 10 plates/bag. Every pack exhibits an irradiation indicator stuck on the side of the bag (8-14 kGy). |
Composition
| Ingredient | g/L |
|---|---|
| D(+)-Glucose | 40.0 |
| Peptone from casein | 5.00 |
| Meat peptone | 5.00 |
| Agar | 15.0 |
| Chloramphenicol | 0.05 |
Quality control
Physical/chemical control
| Color | Pale yellow |
|---|---|
| pH | 5.6 ± 0.2 at 25 °C |
Microbiological control
Growth Promotion Test according to harmonized pharmacopoeial monographs and test methods and ISO 11133:2014.
Spiral spreading: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity).
Aerobiosis. Incubation at 22.5 ± 2.5 °C. Reading at 24-72 h for bacteria and 3-5 days for yeasts and moulds.
| Microorganism | Growth |
|---|---|
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 50%) |
| Aspergillus brasiliensisATCC 16404, WDCM 00053 | Good (≥ 50%) |
| S. cerevisiaeATCC 9763, WDCM 00058 | Good (≥ 50%) |
| Escherichia coliATCC 8739, WDCM 00012 | Inhibited |
| Bacillus subtilisATCC 6633, WDCM 00003 | Inhibited |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
(Base sterilized by autoclaving with continuous stirring at 121 °C for 15 minutes - sterile supplements added at 50 °C.)
Further gamma radiation.
Bibliography
- AJELLO, L. (1957) Cultural Methods for Human Pathogenic Fungi. J. Chron. Dis. 5:545-551.
- EUROPEAN PHARMACOPOEIA 6.0 (2008) 6th ed. § 2.6.13. Microbiological examination of non-sterile products: Test for specified microorganisms. EDQM. Council of Europe. Strasbourg.
- GEORGE, L.K., AJELLO, L. & PAPAGEORGE, C. (1954) Use of Cycloheximide in the Selective Isolation of Fungi Pathogenic to Man. J. Lab. Clin. Med, 44 (422-428).
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 16212 Standard (2017) Cosmetics - Microbiology - Enumeration of yeast and mould.
- PAGANO, J. LEVIN, J.D. & TREJO, W. (1957-58) Diagnostic Medium for Differentiation of Species of Candida. Antibiotics Annual, 137-143.
- SABOURAUD, R. (1910) Les Teignes. Masson, Paris.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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