Ready-to-use media
Sabouraud TLHTh Agar. EP/USP Double Wrapp. Irradiated
Medium with neutralisers for the enumeration and cultivation of fungi, according to harmonized pharmacopoeial monographs and test methods.
- Cat. No.
- LC-838
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
Sabouraud Dextrose Agar is a modification of the classical Sabouraud medium for the cultivation of fungi. This formula helps to maintain the morphology of fungi, providing a reliable medium for both cultivation and differentiation.
Key features
Industry
Environmental monitoring.
Presentation
20 irradiated plates, 90 mm, double wrapping, with 21 ± 2 mL.
Packaging
1 box with 2 cellophane bags (double wrapping) with 10 plates/bag. Every pack exhibits an irradiation indicator stuck on the side of the bag (8-14 kGy).
Description and technique
Description
Its selectivity is due to a low pH and a high glucose concentration, which together with incubation at a relatively lower temperature (25-30 °C) favours the growth of fungi while discouraging that of bacteria.
The mixture of peptones employed has been selected to provide the fungi with all their nitrogen requirements.
The addition of neutralising agents TLHTh (Tween 80 - Lecithin - Histidine - Sodium Thiosulphate) may inactivate a variety of disinfectants.
- The combination of lecithin, polysorbate 80 and histidine neutralises aldehydes and phenolic compounds.
- The combination of lecithin and polysorbate 80 neutralises the quaternary ammonium compounds.
- The polysorbate 80 neutralises hexachlorophene and mercurial derivatives.
- Sodium thiosulphate neutralises halogen compounds.
- Lecithin neutralises chlorhexidine.
- Histidine neutralises formaldehyde.
Technique
Incubate the plates aerobically at 22 ± 2 °C up to 5 days, or at 35 ± 2 °C for 48-72 h.
(Incubation times greater than those mentioned above or different incubation temperatures may be required depending on the sample, on the specifications.)
After incubation, enumerate all the colonies that have appeared on the surface of the membrane.
Calculate total microbial count per mL of sample by multiplying the average number of colonies per plate by the inverse dilution factor. Report results as colony forming units (CFU) per mL along with incubation time and temperature.
Presentation
| Presentation | 20 irradiated plates, 90 mm, double wrapping |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 cellophane bags (double wrapping) with 10 plates/bag. Every pack exhibits an irradiation indicator stuck on the side of the bag (8-14 kGy). |
Composition
| Ingredient | g/L |
|---|---|
| D(+)-Glucose | 40.0 |
| Peptone from casein | 5.0 |
| Meat peptone | 5.0 |
| Lecithin | 0.7 |
| Polysorbate 80 | 5.0 |
| Histidine | 1.0 |
| Sodium thiosulphate·5H₂O | 0.5 |
| Agar | 15.0 |
Quality control
Physical/chemical control
| Color | Straw-coloured yellow |
|---|---|
| pH | 5.6 ± 0.2 at 25 °C |
Microbiological control
Inoculate 10-100 CFU per unit according to the harmonized European Pharmacopoeia.
Aerobiosis. Incubation at 20-25 °C, reading after 24-72 hours for bacteria and 3-5 days for yeasts and moulds.
| Microorganism | Growth |
|---|---|
| Aspergillus brasiliensisATCC 16404, WDCM 00053 | Good (≥ 70%) |
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 70%) |
| Escherichia coliATCC 8739, WDCM 00012 | Good (≥ 50%) - atypical colonial appearance |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- AJELLO, L. (1957) Cultural Methods for Human Pathogenic Fungi. J. Chron. Dis. 5:545-551.
- COLIPA (1997) Guidelines on Microbial Quality Management (MQM). Brussels.
- EUROPEAN PHARMACOPOEIA 8.0 (2014) 8th ed. § 2.6.13. Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. EDQM. Council of Europe. Strasbourg.
- GEORGE, L.K., AJELLO, L. & PAPAGEORGE, C. (1954) Use of Cycloheximide in the Selective Isolation of Fungi Pathogenic to Man. J. Lab. Clin. Med, 44 (422-428).
- HANTSCHKE, D. (1968) Mykosen, 11, (769-778).
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 16212 Standard (2017) Cosmetics - Microbiology - Enumeration of yeast and mould.
- PAGANO, J. LEVIN, J.D. and TREJO, W. (1957-58) Diagnostic Medium for Differentiation of Species of Candida. Antibiotics Annual, 137-143.
- SABOURAUD, R. (1910) Les Teignes. Masson, Paris.
- USP 33 - NF 28 (2011) <62> Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. USP Corp. Inc. Rockville. MD. USA.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
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