Ready-to-use media
Yeast Extract Peptone Dextrose Agar (YPD Agar)
Solid medium for the cultivation of yeast in molecular biology procedures.
- Cat. No.
- LC-834
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- ISO 17025 quality control
- Yes
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Minimum order
- 15 packs
Solid version of the YEPD that supports the growth of most heterotrophic microorganisms. Its simple composition has been adopted as the basal medium for the routine cultivation of yeasts for molecular biology studies. YPD Broth contains peptone as a source of carbon, nitrogen, vitamins and minerals. Yeast extract supplies B-complex vitamins which stimulate bacterial growth. Dextrose is the carbohydrate source. Agar is the solidifying agent.
Key features
Applications
Enrichment of yeasts.
Industry
Molecular biology.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Packaging
1 box with 2 packs of 10 plates/pack. Single cellophane.
Description and technique
This medium can be inoculated directly or after enrichment broth. Proceed according to current regulations or internal specification.
Spread the plates by streaking methodology or by spiral method. Incubate at 25 ± 2 °C for 48-72 hours before enumeration.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Yeast extract | 10.0 |
| Peptone | 20.0 |
| Dextrose | 20.0 |
| Agar | 15.0 |
Quality control
Physical/chemical control
| Color | Light amber |
|---|---|
| pH | 6.5 ± 0.2 at 25 °C |
Microbiological control
Inoculum 100-1000 CFU.
Aerobiosis. Incubation at 25 ± 1 °C, reading at 24-48-72 h to 5 days.
| Microorganism | Growth |
|---|---|
| S. cerevisiaeATCC 9763, WDCM 00058 | Good |
| Candida albicansATCC 10231, WDCM 00054 | Good |
| Saccharomyces cerevisiaeATCC 2601 | Good |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press. Boca Raton. Fla.
- AUSUBEL, F.M., R. BRENT, R.E. KINGSTON, D.D. MOORE, J.G. SEIDMAN, J.A. SMITH & K. STRUHL (1994) Current Protocols in Molecular Biology. Current Protocols. Brooklyn. NY.
- MARTINEZ, J.P., M.L. GIL, M. CASANOVA, J.L. LOPEZ-RIBOT, J. GARCIA de LOMAS & R. SENTANDREU (1990) Wall mannoproteins in the cells from colonial phenotypic variants. J. gen. Microbiol. 136:2421-2432.
- SHERMAN, F. (1991) Studies on the phenotype switching with Candida albicans. Meth. Enzymol. 194:3-17.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
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