Ready-to-use media
Malt Extract with Chloramphenicol Agar
Medium for detection, isolation and enumeration of fungi, particularly yeast and moulds, also from air and water samples.
- Cat. No.
- LC-831
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- ISO 17025 quality control
- Yes
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
Malt Extract + Chloramphenicol Agar promotes the growth of almost all fungi because of its balanced composition, and its ability to inhibit bacteria due to the addition of antibiotic.
Key features
Applications
Selective enumeration and selective isolation of yeasts and molds.
Industry
Water.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Packaging
1 box with 2 packs of 10 plates/pack. Single cellophane.
Description and technique
Collect, dilute and prepare samples and volumes as required according to specifications, directives, official standard regulations and/or expected results.
Spread the plates by streaking methodology or by spiral method. Incubate the plates right side up aerobically at 25-30 °C for up to 5 days.
(Incubation times longer than those mentioned above or different incubation temperatures may be required depending on the sample, on the specifications, etc.)
After incubation, enumerate all the colonies that have appeared on the surface of the agar.
Each laboratory must evaluate the results according to their specifications.
Calculate total microbial count per mL of sample by multiplying the average number of colonies per plate by the inverse dilution factor if a diluted sample was streaked. Report results as colony forming units (CFU) per mL or g along with incubation time and temperature.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Malt extract | 30.00 |
| Soy peptone | 3.00 |
| Chloramphenicol | 0.10 |
| Agar | 15.00 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|---|
| pH | 5.5 ± 0.2 at 25 °C |
Microbiological control
Spiral spreading: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity).
Aerobiosis. Incubation at 22.5 ± 2 °C for up to 5 days for yeast and moulds.
| Microorganism | Growth |
|---|---|
| S. cerevisiaeATCC 9763, WDCM 00058 | Good (≥ 50%) |
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 50%) |
| Aspergillus brasiliensisATCC 16404, WDCM 00053 | Good (≥ 50%) |
| Bacillus subtilisATCC 6633, WDCM 00003 | Inhibited |
| Escherichia coliATCC 8739, WDCM 00012 | Inhibited |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M., L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- BALLOWS, HAUSLER, HERMAN, ISENBERG & SHADOMY (eds.) (1991) Manual of Clinical Microbiology. ASM. Washington.
- DOWNES, F.P. & K. ITO (2001) Compendium of Methods for the Microbiological Examination of Foods. 4th ed. APHA. Washington.
- FDA (Food and Drug Administration) (1978) Bacteriological Analytical Manual A.O.A.C. Washington.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 16212 Standard (2017) Cosmetics - Microbiology - Enumeration of yeast and mould.
- ISO 16000-17:2008 Indoor Air - Detection and enumeration of moulds - Culture Based method.
- RAPP, M. (1974) Indikator-Zusätze zur Keimdifferenzierung auf würze und Malzextrakt Agar. Milchwiss. 29:341-34.
- REIS, J. (1972) Ein selektives kulturmedium für der Nachweiss von Aspergillus flavus. Zbl. Bokt. Hyg. I. Abt. Orig. 220:564-566.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.