Ready-to-use media
Pseudomonas CFC Agar ISO
Selective medium for isolation of Pseudomonas spp. from food samples, according to ISO standards.
- Cat. No.
- LC-829
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- ISO 17025 quality control
- Yes
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Standards
- ISO 13720
Pseudomonas CFC Agar is a selective medium recommended by ISO 13720 for the enumeration of Pseudomonas spp. in meat and meat products, including poultry.
Key features
Applications
Selective enumeration and selective isolation of Pseudomonas.
Industry
Clinical, food.
Standards
ISO 13720.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Description and technique
Description
Gelatin peptone and enzymatic digest of casein provide nitrogen, vitamins, minerals and amino acids essential for growth and permit the growth of a great number of Pseudomonas spp. The potassium sulfate and magnesium chloride help the formation of pigmentation (pyocyanin). The addition of cetrimide, fucidin and cephalothin makes the medium more selective for Pseudomonas spp., including P. aeruginosa and Burkholderia cepacia.
Technique
Collect, dilute and prepare samples and volumes as required according to specifications, directives, official standard regulations and/or expected results.
Spread the plates by streaking methodology or by spiral method.
Incubate the plates right side up aerobically at 24-26 °C for 44 ± 4 h.
(Incubation times longer than those mentioned above or different incubation temperatures may be required depending on the sample, on the specifications, etc.)
After incubation, enumerate all the colonies that have appeared on the surface of the agar.
Each laboratory must evaluate the results according to their specifications.
Presumptive isolation of Pseudomonas spp. must be confirmed by further microbiological or biochemical tests.
Colonies which show a positive oxidase reaction but no glucose fermentation are Pseudomonas spp. colonies.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 cellophane bags with 10 plates/bag. |
Composition
| Ingredient | g/L |
|---|---|
| Gelatine pancreatic peptone | 16.0 |
| Casein hydrolysate | 10.0 |
| Potassium sulphate | 10.0 |
| Magnesium chloride | 1.40 |
| Agar | 11.0 |
| Cetrimide | 0.01 |
| Fucidin | 0.01 |
| Cephalothin sodium salt | 0.05 |
| Glycerol | 10.0 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|---|
| pH | 7.2 ± 0.2 at 25 °C |
Microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ (selectivity).
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobiosis. Incubation at 24-26 °C, reading after 40-48 hours.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 8739, WDCM 00012 | Inhibited |
| Ps. fluorescensATCC 13525, WDCM 00115 | Good (≥ 50%) |
| Ps. fragiNCTC 10689 | Good (≥ 50%) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- BROWN, V.L. & E.J.L. LOWBURY (1965) Use of an improved Cetrimide Agar Medium and of culture methods for P. aeruginosa. J. Clin. Pathol. 18:752.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- EN 12780 Standard (2002) Water Quality. Detection and enumeration of P. aeruginosa by membrane filtration.
- ISO 13720 Standard (2010) Meat and meat products. Enumeration of presumptive Pseudomonas spp.
- GOTO S. & S. ENOMOTO (1970) Nalidixic acid cetrimide agar. A new selective plating medium for the selective isolation of P. aeruginosa. Jpn. J. Microbiol. 14:65.
- ISO 16266 Standard (2006) Water Quality - Detection and enumeration of Pseudomonas aeruginosa - Method by membrane filtration.
- KING, E.O., M.K. WARD & E.E. RANEY (1954) Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44:301.
- ROBIN, T. & J.M. JANDA (1984) Enhanced recovery of P. aeruginosa from diverse clinical specimens on a new selective agar. Diag. Microbiol. Infect Dis. 2:207.
- SCHWEIZERISCHES LEBENSMITTELBUCH (2005) Kap. 56 Mikrobiologie. Bundesamt für Gesundheit. Direktionsbereich Verbraucherschutz. Bern.
- ISO/TS 11059:2009. Milk and milk products - Method for the enumeration of Pseudomonas spp.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
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