Ready-to-use media
Malt Extract Agar Acidified (pH 4,3)
Medium for detection, isolation and enumeration of fungi, particularly yeast and moulds, also from air and water samples.
- Cat. No.
- LC-821
- Category
- Ready-to-use media
- Formats
- 3 formats
- Minimum order
- 15 packs
Malt Extract Agar may support the growth of almost all of the fungi very well, because of its balanced composition, and restrains most of the bacteria due to the strong acidity.
Key features
Applications
Detection and selective enumeration of yeasts and molds.
Industry
Water.
Testing
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Description and technique
Technique
Collect, dilute and prepare samples and volumes as required according to specifications, directives, official standard regulations and/or expected results.
Incubate the plates right side up aerobically at 20-25 °C for up to 5/7 days.
(Incubation times longer than those mentioned above or different incubation temperatures may be required depending on the sample, on the specifications, etc.)
After incubation, enumerate all the colonies that have appeared on the surface of the agar.
Each laboratory must evaluate the results according to their specifications.
Calculate total microbial count per mL of sample by multiplying the average number of colonies per plate by the inverse dilution factor if a diluted sample was streaked. Report results as colony forming units (CFU) per mL or g along with incubation time and temperature.
LC-4550, 55 mm plates for filtration
Should more selection against the bacterial growth be desired, readjust the pH to 3.5.
LC-4071 and LC-4550, tubes and 55 mm plates
Filter the sample through a 0.45 µm pore membrane and apply it onto the surface of the agar.
Presentation
| Code | Presentation | Filling | Packaging |
|---|---|---|---|
| LC-821 | 20 prepared plates, 90 mm | 21 ± 2 mL | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
| LC-4071 | 20 tubes, 17 x 145 mm | 20 ± 5 mL | 17 x 145 mm glass tubes, ink labelled, metal non-injectable cap. 20 tubes per box. |
| LC-4550 | 30 prepared plates, 55 mm, for filtration purposes | 9 ± 1 mL | 1 box containing 6 plastic bags with 5 plates of 55 mm/bag. |
Composition
| Ingredient | g/L |
|---|---|
| Malt extract | 30.00 |
| Soy peptone | 3.00 |
| Agar | 15.00 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|---|
| pH | 4.3 ± 0.2 at 25 °C |
Microbiological control
Membrane filtration: practical range 100 ± 20 CFU, min. 50 CFU (productivity).
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobic. Incubation at 22.5 ± 2 °C, 3-5 days (moulds and yeast).
| Microorganism | Growth |
|---|---|
| Aspergillus brasiliensisATCC 16404 | Good (≥ 70%) |
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 70%) |
LC-4071, tubes: microbiological control
Melt the medium - prepare plates - transfer the filtration membrane.
Membrane filtration: practical range 100 ± 20 CFU, min. 50 CFU (productivity).
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobic. Incubation at 22.5 ± 2 °C, 3-5 days (moulds and yeast).
| Microorganism | Growth |
|---|---|
| Aspergillus brasiliensisATCC 16404 | Good (≥ 70%) |
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 70%) |
LC-4550, 55 mm plates: microbiological control
Membrane filtration: practical range 100 ± 20 CFU, min. 50 CFU (productivity).
Aerobiosis. Incubation at 22.5 ± 2 °C for up to 5 days for yeast and moulds.
| Microorganism | Growth |
|---|---|
| Aspergillus brasiliensisATCC 16404 | Good (≥ 70%) |
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 70%) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M., L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- BALLOWS, HAUSLER, HERMAN, ISENBERG & SHADOMY (eds.) (1991) Manual of Clinical Microbiology. ASM. Washington.
- DOWNES, F.P. & K. ITO (2001) Compendium of Methods for the Microbiological Examination of Foods. 4th ed. APHA. Washington.
- FDA (Food and Drug Administration) (1978) Bacteriological Analytical Manual A.O.A.C. Washington.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 16000-17:2008 Indoor Air - Detection and enumeration of moulds - Culture Based method.
- RAPP, M. (1974) Indikator-Zusätze zur Keimdifferenzierung auf würze und Malzextrakt Agar. Milchwiss. 29:341-34.
- REIS, J. (1972) Ein selektives kulturmedium für der Nachweiss von Aspergillus flavus. Zbl. Bokt. Hyg. I. Abt. Orig. 220:564-566.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.