Ready-to-use media
Clostridium Perfringens Agar (m-CP)
Solid medium for the enumeration and isolation of Clostridium perfringens in water according to the European Directive 12767/97.
- Cat. No.
- LC-4709
- Category
- Ready-to-use media
- Format
- 30 water plates, 55 mm
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Standards
- Council Directive 98/83/EC
The m-CP Agar Base is a solid medium for counting and isolating vegetative cells and spores of Clostridium perfringens by the membrane filtration method. Its use is compulsory in determining the quality of water for human consumption according to the European Union, by Directive 12767 (12-07-1997) of the European Council.
Key features
Applications
Selective enumeration and isolation of Clostridium perfringens.
Industry
Water.
Standards
Council Directive 98/83/EC.
Presentation
30 prepared plates, 55 mm, for filtration purposes, with 9 ± 1 mL.
Description and technique
Technique
A suitable volume of water is filtered through a membrane filter of 47 mm diameter and 0.45 µm pore. The membrane is then placed on the surface of freshly prepared m-CP medium and incubated in an anaerobic atmosphere at 44 ± 1 °C for 21 ± 3 hours. Expose the growth obtained to ammonium hydroxide vapours for 20-30 seconds. Count as Clostridium perfringens all the opaque yellow colonies that turn pink or red after ammonium hydroxide exposure. Express the results as CFU/mL.
Presentation
| Presentation | 30 prepared plates, 55 mm, for filtration purposes |
|---|---|
| Filling | 9 ± 1 mL |
| Packaging | 1 box containing 5 plastic bags with 6 plates of 55 mm/bag. |
Composition
| Ingredient | g/L |
|---|---|
| Tryptose | 30.000 |
| Yeast extract | 20.000 |
| Sucrose | 5.000 |
| L-Cysteine HCl | 1.000 |
| Magnesium sulfate·7H₂O | 0.100 |
| Bromocresol purple | 0.040 |
| D-Cycloserine | 0.400 |
| Polymyxin B sulfate | 0.025 |
| 3-Indoxyl-beta-D-glucopyranoside | 0.060 |
| Phenolphthalein biphosphate 0.5% | 20 mL |
| Fe(III) chloride solution 4.5% | 2 mL |
| Agar | 15.000 |
Quality control
Physical/chemical control
| Color | Violet |
|---|---|
| pH | 7.6 ± 0.2 at 25 °C |
Microbiological control
Membrane filtration / practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ CFU for selectivity.
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Anaerobiosis. Incubation at 44 ± 1 °C for 21 ± 3 h.
| Microorganism | Growth |
|---|---|
| Clostridium perfringensATCC 13124, WDCM 00007, NCTC 8237 | Good - opaque yellowish colonies |
| Clostridium perfringensATCC 10543, WDCM 00174 | Good - opaque yellowish colonies |
| Escherichia coliATCC 25922, WDCM 00013 | Inhibited |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- European Council (1997) Directive 12767/97 on the quality of water destined for human consumption. EC Bull. 16-12-1997.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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