Ready-to-use media
Linden Grain Broth
Liquid medium for media fill process simulation for beverage bottling, to test for low acid beverage spoiling microorganisms.
- Cat. No.
- LC-4697
- Category
- Ready-to-use media
- Format
- 10 x 450 mL flasks
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 4 months
The Linden Grain Broth is designed for media fill process simulation for beverage bottling (also known as Aseptic Conditioning Testing) in the modern beverage industry. The medium permits the growth of organisms that can spoil low acid beverages.
Key features
Presentation
10 prepared bottles, 500 mL, with 450 ± 5 mL.
Packaging
1 box with 10 bottles 500 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended.
Testing
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Description and technique
The presence of spoiling microorganisms is indicated by turbid growth in the broth, after the incubation in the standardized conditions for every industry.
Use the medium according to intended purpose, samples and validated methods.
Presentation
| Presentation | 10 prepared bottles, 500 mL |
|---|---|
| Filling | 450 ± 5 mL |
| Packaging | 1 box with 10 bottles 500 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
Composition
| Ingredient | g/L |
|---|---|
| D(+)-Glucose | 20.00 |
| Yeast extract | 3.50 |
| Casein peptone | 2.00 |
| Ammonium sulfate | 2.00 |
| Magnesium sulfate | 1.00 |
| Potassium dihydrogen phosphate | 1.00 |
PROTECT FROM LIGHT AT ALL TIMES. AVOID PROLONGED EXPOSURE TO LIGHT.
Quality control
Physical/chemical control
| Color | Pale yellow |
|---|---|
| pH | 4.3 ± 0.2 at 25 °C |
Microbiological control
Prepare tubes - inoculate: practical range 100 ± 20 CFU, min. 50 CFU (productivity).
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobiosis. Incubation at 25 ± 1 °C, reading at 72 h to 5 days.
| Microorganism | Growth |
|---|---|
| Saccharomyces cerevisiaeATCC 9763 | Good |
| Aspergillus brasiliensisATCC 16404 | Good |
| Candida albicansATCC 10231, WDCM 00054 | Good |
| Dekkera spp. | Good |
| Dekkera bruxellensisATCC 36234 | Good |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- SYPOSS, Z. (2003) The role of risk analysis in the Food Quality & Safety Management Systems. Ph. D. Thesis. Department of Postharvest. Faculty of Food Sciences. Szent István University. Budapest.
- SYPOSS, Z. & J. TORNAI-LEHOCZKI (2003) Application of acidified (pH 4,5) Linden Grain Medium as a microbiological validation tool in the Aseptic Beverage PET Technology. 23rd International Specialized Symposium on Yeasts (ISSY 23). Budapest, Hungary. Food Microbiology 86(1-2):2003:1-212.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.